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| celB基因标记法检测苜蓿根瘤菌竞争性的可行性研究 | |
| 引用本文： | 钟文文,张小平. celB基因标记法检测苜蓿根瘤菌竞争性的可行性研究[J]. 安徽农业科学, 2006, 34(7): 1311-1312,1320 |
| 作者姓名： | 钟文文 张小平 |
| 作者单位： | 1. 临沂师范学院,山东临沂,276001 2. 四川农业大学资源环境学院,四川雅安,625014 3. 芬兰赫尔辛基大学应用化学与微生物系,Fin-00014 |
| 基金项目： | 科技部科研项目 , 芬兰赫尔辛基大学校科研和校改项目 |
| 摘 要： | 对celB基因标记法检测苜蓿根瘤菌竞争性的可行性进行了研究。通过供体大肠杆菌HAMBI2356与根瘤菌接合的方法成功地将celB基因导入到4株苜蓿根瘤菌株Y611、BB1811、BB2211和WZ622中,然后对标记基因可能会对标记菌株产生的影响进行了一系列检测,结果表明标记基因在标记菌株内能稳定传代,而且对标记菌株的生长、竞争性及有效性都没有显著影响,从而证明celB基因标记技术可以作为一种简便、直观而又有效的检测手段用于追踪根瘤菌株在土壤中的竞争性。 |
| 关 键 词： | 苜蓿 根瘤菌 筛选 celB基因 竞争性 有效性 |
| 文章编号： | 0517-6611（2006）07-1311-02 |
| 收稿时间： | 2006-01-20 |
| 修稿时间： | 2006-01-20 |
Study on the Feasibility of Competitiveness of Rhizobium with celB Gene |
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| Kristina Lindstrom. Study on the Feasibility of Competitiveness of Rhizobium with celB Gene[J]. Journal of Anhui Agricultural Sciences, 2006, 34(7): 1311-1312,1320 | |
| Authors: | Kristina Lindstrom |
| Affiliation: | Linyi Normal University, Linyi,Shandong 276001 |
| Abstract: | The aim of this experiment was to study the feasibility of the competitiveness of rhizobium melioti strains with celB gene.By the conjugation with Escherichia coli HAMBI2356 which contained the celB gene,the celB gene were successfully introduced into four rhizobial strains of alfalfa,Y611,BB_1811,BB_2211and WZ622 respectively.The stability and impact of the insert celB gene on the rhizobial strains were evaluated by the determination of generation time and pot inoculation test.The results showed that the marker gene was highly stable,and had no significant effect on the growth,competitiveness and symbiotic effectiveness of the rhizobial strains.So the celB gene marker technique can be used as a perfect means to study the competition of the rhizobial strains of alfalfa. |
| Keywords: | Rhizobium Alfalfa celB marker gene Competition |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |