复合酶法澄清葡萄籽提取液工艺研究

[目的]对葡萄渣提取液进行澄清,并对工艺参数进行优化.[方法]选择果胶和纤维素复合酶对葡萄籽提取物进行澄清,分别在最适温度和常温作用、最适pH和不调pH条件下此较了复合酶对葡萄籽提取液澄清效果及多酚和原花青素含量的影响.[结果]试验表明,复合酶对葡萄籽提取液澄清效果受温度的影响较大,调节pH对澄清度的影响则表现不明显.正交试验证明,最佳澄清工艺条件为pH 5.5,酶解温度45℃、作用时间6h、酶剂量60 mg/kg,透光率达到76％,澄清过程中不影响葡萄籽多酚以及原花青素含量.[结论]研究可为葡萄籽提取液的开发利用提供理论依据.

Clarified Grape Seed Extract by Combined-enzyme Method

[Objective] To clarify grape seed extract and optimize process parameters.[Methods] Selected fpectin and cellulose complex enzyme for clarification effect of grape seed extract,at the appropriate temperature and room temperature effects,and non-optimal pH,appropriate pH,and compared clarification effect of grape seed extract and polyphenols and proanthocyanidins efficiency levels of complex enzyme.[Results] The results showed the effect of enzyme clarifying the grape seed extract was greatly influenced by the temperature; the effect of the adjusted pH was not significant on the clarification result.Orthogonal showed the Best clarification process condition is pH 5.5,reaction temperature 45 ℃,reaction time for 6 h,enzyme dose 60 mg/kg.Transmittance efficiency reaches 76％ and the process does not affect the content of grape seed polyphenols and proanthocyanidins.[Conclusion] This study can provide certain basis for development and application of grape seed extract.