Use of ELISA to detect toxigenic Pasteurella multocida in atrophic rhinitis in swine. |
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| Authors: | T L Bowersock T Hooper R Pottenger |
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| Institution: | Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, W. Lafayette, IN 47907-1243. |
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| Abstract: | The use of an enzyme-linked immunosorbent assay (ELISA) as a means of detecting dermonecrotoxin-producing strains of Pasteurella multocida was investigated. The assay was evaluated as a means to identify toxigenic P. multocida isolates recovered from nasal secretions of swine with atrophic rhinitis. The sensitivity and specificity of the ELISA for detecting dermonecrotoxin-producing P. multocida strains were compared to those of mouse-inoculation and cytotoxicity assays. The ELISA was highly sensitive and more specific than animal inoculation or tissue culture assay and is thus a more effective method for screening swine herds for the presence of toxigenic strains of P. multocida. The ELISA is a rapid, effective, economical way to identify toxigenic P. multocida isolates. |
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