| 裙带菜凝集素的分离及性质研究 |
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| 引用本文： | 李丹彤,崔铁军,赵元凤,许庆陵,郝玉.裙带菜凝集素的分离及性质研究[J].大连水产学院学报,2002,17(2):84-88. |
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| 作者姓名： | 李丹彤 崔铁军 赵元凤 许庆陵 郝玉 |
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| 作者单位： | 大连水产学院养殖系,辽宁,大连,116023 |
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| 基金项目： | 农业部八五青年基金项目(渔85－93－青05) |
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| 摘 要： | 裙带菜（Undaria pinnatifida)经Tris-HCl缓冲液粗抽提，然后进行硫酸铵分级实验，测定盐析范围为50％硫酸铵饱和度，经50％硫酸铵饱和度分级后，裙带菜凝集素（UPL）纯化倍数为7．7，总活力回收为167％，UPL粗抽提液中糖的质量分数为5．72％。该凝集素在pH为4．0－10．14时均为活性，但在pH为5．0－9．51时活性较高，pH等于4．0时活力保留25％，pH等于10．14时活力保留50％，该凝集素不被D－果糖，葡萄糖，γ－球蛋白所抑制。
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| 关 键 词： | 分离 性质 裙带菜 凝集素 血凝活力 |
| 文章编号： | 1000-9957(2002)02-0084-05 |
Isolation and properties of lectin from Undaria pinnatifita |
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| LI Dan-tong,CUI Tie-jun,ZHAO Yuan-feng,XU Qing-ling,HAO Yu.Isolation and properties of lectin from Undaria pinnatifita[J].Journal of Dalian Fisheries University,2002,17(2):84-88. |
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| Authors: | LI Dan-tong CUI Tie-jun ZHAO Yuan-feng XU Qing-ling HAO Yu |
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| Abstract: | The lectin from brown algae Undaria pinnatifida was isolated by extraction withTBS, followed by 50% ammonium sulfate fractionation. The lectin was purified by 7.7 times,and had 167% recycle - rate. It contained 5. 72% of sugar in crude extract. The hemaggluti-nating activity for rabbit could not be inhibited by D -fructose, glucose, gamma - globulin (hu-man). This lectin had the maximum hemagglutinating activity at pH 5. 0 ~9. 51, with activityat pH 4. 0~10. 14. |
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| Keywords: | Undaria pinnatifida lectin hemagglutinating activity |
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