假型病毒的应用研究进展

一种病毒的核酸被另一种病毒编码的包膜蛋白所包被,称为假型病毒。假型病毒在细胞内只能进行单一周期复制,安全性好,并具有广泛的宿主范围,更高的转染效率,更容易浓缩成高滴度,能抵抗血清补体的攻击,非细胞周期依赖性地高效转染静止细胞等优点,因而假型病毒在研究病毒进入过程、受体鉴定、中和抗体检测、疫苗评价等方面具有重要作用。

Progress on Pseudotyped virus in Application

A Pseudotyped virus consists of two parts: envelope protein from one virus which usually plays the role of receptor binding ligand for entering target cells,and nucleoprotein and viral genome from a second virus which usually forms the backbone of the pseudotyped virus,and replication within target cells.Pseudotyped virus has the unique characteristic of single-round replication,therefore,it provides super safety when used in research.Because of its replaceable multi-viral envelop,pseudotyped virus can infect a wide range of target cells,transfect target cells with higher efficiency,be concentrated easily to higher titers compared with its native outer shell virus,resist damage against serum complements,and transfect quiescent cells efficiently in independence of cell-cycles.Pseudotyped viruses have been applied widely in the field of life science research,including the investigation of virus entry pathways,the identification of virus receptors,the qualitative and quantitative analysis of neutralizing antibodies,and the evaluation of virus vaccines.