| 柿花发育相关的MADS2box基因克隆与表达 |
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| 引用本文： | 丁燕,韩振海,许雪峰,李天忠.柿花发育相关的MADS2box基因克隆与表达[J].园艺学报,2007,34(1):39-42. |
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| 作者姓名： | 丁燕 韩振海 许雪峰 李天忠 |
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| 作者单位： | (中国农业大学园艺植物研究所, 北京100094) |
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| 摘 要： | 以雌花型柿(Diospyros kaki) ‘阳丰’花为试材, 克隆得到一个与其花发育相关的MADS2box基因, 命名为DkMADS1, GenBank登录号为DQ412058。该基因cDNA全长1 193 bp, ORF为747 bp, 编码一个含有249个氨基酸的蛋白。DkMADS1 具保守的MADS区及半保守的K区, 与其它植物中的MADS2box蛋白有很高同源性。RT-PCR表达分析表明, 该基因在‘阳丰’花的萼片、花瓣、子房和‘禅寺丸’的雄蕊中均有表达。
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| 关 键 词： | 柿 花 基因 克隆 表达 |
| 文章编号： | 0513-353X（2007）01-0039-04 |
| 收稿时间： | 2006-04-18 |
| 修稿时间： | 2006-04-182006-10-27 |
Molecular Cloning and Expression of Flowering-related MADS-box Gene in Diospyros kaki |
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| DING Yan,HAN Zhen-hai,XU Xue-feng,LI Tian-zhong.Molecular Cloning and Expression of Flowering-related MADS-box Gene in Diospyros kaki[J].Acta Horticulturae Sinica,2007,34(1):39-42. |
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| Authors: | DING Yan HAN Zhen-hai XU Xue-feng LI Tian-zhong |
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| Institution: | ( Institute of Horticultural Plants, China Agricultural University, Beijing 100094, China) |
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| Abstract: | A MADS-box gene named DkMADS1 was cloned from Yangfeng persimmon flowers(GenBank accession No.DQ412058).The sequence of complete DkMADS1 cDNA gene is 1 193 bp,with an ORF encoding 249 amino acids.The deduced protein contained conservative MADS domain and semi-conservative K domain.It shared highly homology to other MADS-box proteins from different species.RT-PCR analysis showed that DkMADS1 gene was expressed in sepal,petal,ovary of Yangfeng persimmon and stamen of Zenjimaru persimmon. |
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| Keywords: | Persimmon Diospyros kaki Flower Gene Cloning Expression |
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