| 基于DNA条形码的广西苦瓜中实蝇幼虫分子鉴定研究* |
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| 引用本文： | 姜帆,刘佳琪,李志红,吴佳教,赵朔.基于DNA条形码的广西苦瓜中实蝇幼虫分子鉴定研究*[J].植物保护,2011,37(4):150-153. |
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| 作者姓名： | 姜帆 刘佳琪 李志红 吴佳教 赵朔 |
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| 作者单位： | 1. 中国农业大学农学与生物技术学院,北京,100193
2. 广东出入境检验检疫局检验检疫技术中心,广州,510623 |
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| 基金项目： | 农业部"引进国际先进农业科学技术"项目，国家公益性行业(农业)专项，国家自然科学基金 |
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| 摘 要： | 实蝇是多种热带、亚热带水果和蔬菜的重要害虫，其卵、幼虫、蛹难以通过形态特征进行种类鉴定。DNA条形码是近年来出现的物种分子鉴定技术，能够实现对昆虫非成熟虫态的快速鉴定。本研究利用DNA条形码技术和BOLD系统，选取线粒体DNA细胞色素氧化酶I（COI）基因片段，针对采自于广西南宁地区苦瓜中的实蝇幼虫样品进行了序列测定、比对和分析。结果显示，在所检测的10头实蝇幼虫样品中，有4头为瓜实蝇［Bactrocera cucurbitae (Coquillett)］、6头为南亚果实蝇［Bactrocera tau (Walker)］。
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| 关 键 词： | 实蝇 幼虫 DNA条形码 COI基因 分子鉴定 |
Molecular identification of fruit fly larvae by DNA barcodes |
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| Jiang Fan,Liu Jiaqi,Li Zhihong,Wu Jiajiao,Zhao Shuo.Molecular identification of fruit fly larvae by DNA barcodes[J].Plant Protection,2011,37(4):150-153. |
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| Authors: | Jiang Fan Liu Jiaqi Li Zhihong Wu Jiajiao Zhao Shuo |
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| Institution: | Jiang Fan1,Liu Jiaqi1,Li Zhihong1,Wu Jiajiao2,Zhao Shuo1(1.College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China,2.Inspection and Quarantine Technology Center,Guangdong Entry-Exit Inspection and Quarantine Bureau,Guangzhou 510623,China) |
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| Abstract: | Fruit fly(Diptera:Tephritidae) is an important pest of a variety of tropical and subtropical fruits and vegetables,while species identification at its egg,larvae and adult stages is difficult by morphological characteristics.Emerging as a promising molecular technology of species identification,DNA barcodes can realize rapid identification of the immatures.Based on the DNA barcoding and BOLD system,a series of studies,including sequencing,alignment and analysis,were conducted based on the mitochondrial cyto... |
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| Keywords: | fruit fly larva DNA barcode COI gene molecular identification |
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