Sugars Profiles of Different Chestnut (Castanea sativa Mill.) and Almond (Prunus dulcis) Cultivars by HPLC-RI |
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Authors: | João C M Barreira José Alberto Pereira M Beatriz P P Oliveira Isabel C F R Ferreira |
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Institution: | 1.CIMO-Escola Superior Agrária, Instituto Politécnico de Bragan?a,Bragan?a,Portugal;2.REQUIMTE/Servi?o de Bromatologia, Faculdade de Farmácia da Universidade do Porto,Porto,Portugal |
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Abstract: | Sugar profiles of different almond and chestnut cultivars were obtained by high-performance liquid chromatography (HPLC),
by means of a refractive index (RI) detector. A solid-liquid extraction procedure was used in defatted and dried samples.
The chromatographic separation was achieved using a Eurospher 100-5 NH2 column using an isocratic elution with acetonitrile/water (70:30, v/v) at a flow rate of 1.0 ml/min. All the compounds were separated in 16 min. The method was optimized and proved to be reproducible
and accurate. Generally, more than 95% of sugars were identified for both matrixes. Sugars profiles were quite homogeneous
for almond cultivars; sucrose was the main sugar (11.46 ± 0.14 in Marcona to 22.23 ± 0.59 in Ferragnes g/100 g of dried weight), followed by raffinose (0.71 ± 0.05 in Ferraduel to 2.11 ± 0.29 in Duro Italiano), glucose (0.42 ± 0.12 in Pegarinhos two seeded to 1.47 ± 0.19 in Ferragnes) and fructose (0.11 ± 0.02 in Pegarinhos two seeded to 0.59 ± 0.05 in Gloriette). Commercial cultivars proved to have higher sucrose contents, except in the case of Marcona. Nevertheless, chestnut cultivars revealed a high heterogeneity. Sucrose was the main sugar in Aveleira (22.05 ± 1.48), Judia (23.30 ± 0.83) and Longal (9.56 ± 0.91), while glucose was slightly prevalent in Boa Ventura (6.63 ± 0.49). The observed variance could serve for inter-cultivar discrimination. |
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