| 7个棉花品种SSR位点纯合度研究与分析 |
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| 引用本文： | 王飞,匡猛,许红霞,王延琴,周大云,冯新爱,方丹,邢要非,杨伟华.7个棉花品种SSR位点纯合度研究与分析[J].棉花学报,2013,25(3):234-239. |
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| 作者姓名： | 王飞 匡猛 许红霞 王延琴 周大云 冯新爱 方丹 邢要非 杨伟华 |
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| 作者单位： | 中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南安阳,455000 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项重点项目，农业部农产品质量安全监管(种子管理)项目，国家科技支撑计划 |
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| 摘 要： | 采用52对核心引物对7个棉花常规种进行了SSR分析。结果显示：每个品种的24个受检单粒棉种中，杂粒数最多为9个，最少1个，平均为3.9个；DNA位点纯合率最高为100%，最低92.3%，平均为97.0%。研究表明，品种纯度与DNA位点纯合率呈高度正相关。采用SSR标记进行分子检测，能准确区分因遗传因素与生产加工对品种纯度造成的影响。
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| 关 键 词： | 棉花常规种 SSR标记 纯度 位点纯合率 |
| 收稿时间： | 2013-01-04; |
SSR Locus Purity Research and Analysis of Seven Cotton Cultivars |
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| WANG Fei,KUANG Meng,XU Hong-xia,WANG Yan-qin,ZHOU Da-yun,FENG Xin-ai,FANG Dan,XING Yao-fei,YANG Wei-hua.SSR Locus Purity Research and Analysis of Seven Cotton Cultivars[J].Cotton Science,2013,25(3):234-239. |
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| Authors: | WANG Fei KUANG Meng XU Hong-xia WANG Yan-qin ZHOU Da-yun FENG Xin-ai FANG Dan XING Yao-fei YANG Wei-hua |
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| Institution: | Institute of Cotton Research, Chinese Academy of Agriculture Sciences / State Key Laboratory of Cotton Biology, Anyang, Henan 455000, China |
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| Abstract: | In an SSR study of seven conventional cotton cultivars, we analyzed 24 seeds of each cultivar using 52 core primer pairs. Among the seven cultivars, the number of miscellaneous seeds ranged from 1 to 9, and averaged 3.9. The percentage of homozygous DNA loci varied from 92.3% to 100%, and averaged 97.0%. Our analysis revealed that varietal purity and DNA locus homozygosity levels were highly positively correlated. Using SSR molecular markers, we were able to accurately differentiate between the impacts of genetic factors and seed production procedures on cultivar purity. |
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| Keywords: | SSR marker purity homozygous DNA locus |
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