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| 转鲑鱼降钙素基因酵母对体外培养破骨细胞的影响 | |
| 引用本文： | 姜勇,张辉,王鹏,赵喜喜,徐科凤,王健,张学成. 转鲑鱼降钙素基因酵母对体外培养破骨细胞的影响[J]. 山东农业科学, 2011, 0(6): 99-103 |
| 作者姓名： | 姜勇 张辉 王鹏 赵喜喜 徐科凤 王健 张学成 |
| 作者单位： | 1. 青岛国家海洋科学研究中心,山东青岛,266071 2. 中国农村技术开发中心,北京,100045 3. 山东省农业科学院,山东济南,250100 4. 中国海洋大学,山东青岛,266003 |
| 基金项目： | 国家“863”高技术发展与研究计划项目 |
| 摘 要： | 用机械分离的方法获得新生Wistar大鼠的破骨细胞,在培养液中分别加入经肠激酶酶切的转鲑鱼降钙素基因酵母的上清液和密钙息（Miacalcic,鲑鱼降钙素注射液）,以HE染色观察破骨细胞形态,Image-Pro Plus图像软件分析骨片上骨吸收陷窝的面积,骨片培养7天,吸收陷窝结果显示,转鲑鱼降钙素对破骨细胞吸收功能具有明显的抑制作用。 |
| 关 键 词： | 鲑鱼降钙素 破骨细胞 细胞培养 |
Effect of Recombinant Saccharomyces cerevisiae Expressing Salmon Calcitonin on Osteoclast Culture in vitro |
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| JIANG Yong,ZHANG Hui,WANG Peng,ZHAO Xi-xi,XU Ke-feng,WANG Jian,ZHANG Xue-cheng. Effect of Recombinant Saccharomyces cerevisiae Expressing Salmon Calcitonin on Osteoclast Culture in vitro[J]. Shandong Agricultural Sciences, 2011, 0(6): 99-103 | |
| Authors: | JIANG Yong ZHANG Hui WANG Peng ZHAO Xi-xi XU Ke-feng WANG Jian ZHANG Xue-cheng |
| Affiliation: | (1.National Oceanographic Center in Qingdao,Qingdao 266071,China;2.China Rural Technology Development Center, Beijing 100045,China;3.Shandong Academy of Agricultural Sciences,Jinan 250100,China; 4.Ocean University of China,Qingdao 266003,China) |
| Abstract: | The osteoclasts of new born Wistar mice were obtained by mechanical separation.The recombinant Saccharomyces cerevisiae expressing salmon calcitonin and restricted by enterokinase and Miacalcic were added into the culture solution respectively.The osteoclasts were observed by HE staining.After cultured for seven days,the area of bone resorption pits was analyzed by Image-Pro Plus image software.The results showed that the recombinant Saccharomyces cerevisiae expressing salmon calcitonin had obvious inhibitory action to the absorption function of osteoclasts. |
| Keywords: | Salmon calcitonin Osteoclast Cell culture |
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