禽脑脊髓炎的调查及病毒分离

对陕西省宝鸡、渭南等地的24个鸡群进行了禽脑脊髓炎（AE）的调查,所调查鸡群都有产蛋下降史或产蛋正在下降,下降幅度平均为17.2%。用琼扩试验（AGP）检测所采集的256份血清,AE阳性率平均高达85.6%。有10个鸡群阳性率达100%。跟踪调查2个种鸡群,发现孵出的雏鸡从3日龄开始发病,而且有AE的症状,采发病雏鸡的脑组织,接种6龄鸡胚分离病毒,连传3代,琼扩检测分离毒,呈AE抗原阳性,人工感染1日龄雏鸡复制出与原发病鸡群相同的疾例,表明分离毒是AE病毒。

Investigation and Virus Isolation of Avian Encephalomyelitis

Avian encephalomyelitis(AE) in 24 groups of chi ck en from Baoji and Weinan regions of Shaanxi province was investigated.All stocks involved had the history of the disease or a decreasing egg laying with average reducing extent of 17.2%. 256 serum samples from the stocks were tested with Ag ar Gel precipitin(AGP) test.It showed that 85.6% of the chickens were AE pos itive in average,especially 100% in 10 stocks.Further detection in 2 groups of b r eeding chickens indicated that the chicken appeared the clinical symptoms of AE in 3 days after hatched.The brain sample preperations from the sick chicken were inje cted into chicken embryos to isolate the virus.The virus collected from the thir d generation were tested to be positive for AE using AGP test. 1 day chickens i noculated with the isolates revealed the same symptoims as the natural disease. The result indicated that the isolate should be the AE virus.