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山羊血清可溶性CD58存在的研究
引用本文:马勇江,靳亚平,曹斌云,李引乾,沈文正. 山羊血清可溶性CD58存在的研究[J]. 西北农业学报, 2001, 10(2): 9-12
作者姓名:马勇江  靳亚平  曹斌云  李引乾  沈文正
作者单位:西北农林科技大学畜牧兽医学院
基金项目:国家自然科学基金资助项目(39970544).
摘    要:进行了正常山羊血清对兔 RBC的溶血试验和猪 PBMC Et玫瑰花环试验。根据山羊血清经 SRBC吸收和添加山羊红细胞 CD58后对兔 RBC的破坏作用显著降低 ,认为山羊血清破坏 RBC的作用与血清中游离 CD2有关 ;经透析、反复解离 ,SRBC吸收后的山羊血清可抑制对兔 RBC的破坏作用和猪 PBMC Et玫瑰花环的形成 ,据此 ,进一步认为山羊血清中存在可溶性 CD58

关 键 词:山羊血清  游离CD2  可溶性CD58
文章编号:1004-1389(2001)02-0009-04
收稿时间:2000-02-11

Study on Existing of Soluble CD58 in Goat Serum
MA Yong-jiang,JIN Ya-ping,CAO Bin-yun,LI Yin-qian and SHEN Wen-zheng. Study on Existing of Soluble CD58 in Goat Serum[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2001, 10(2): 9-12
Authors:MA Yong-jiang  JIN Ya-ping  CAO Bin-yun  LI Yin-qian  SHEN Wen-zheng
Affiliation:College of Animal Husbandry and Veterinary Medicine, NWSUAF, Yangling Shaanxi 712100, China;College of Animal Husbandry and Veterinary Medicine, NWSUAF, Yangling Shaanxi 712100, China;College of Animal Husbandry and Veterinary Medicine, NWSUAF, Yangling Shaanxi 712100, China;College of Animal Husbandry and Veterinary Medicine, NWSUAF, Yangling Shaanxi 712100, China;College of Animal Husbandry and Veterinary Medicine, NWSUAF, Yangling Shaanxi 712100, China
Abstract:The experiments of the haemolytic of goat serum to rabbit RBC (RRBC) and pig PBMC Et rosette have been studied. When goat serum was absorbed by SRBC or added CD58 derived from goat RBC (GRBC), the haemolzation of it to SRBC was reduced significantly. These results suggest that the haemolysis of goat serum to RRBC is related to the existing of free form CD2 in the serum.In addition, the goat serum could block the haemolysis of SRBC and the formation of pig PBMC Et rosette after it was dissociated and absorded by SRBC. It suggests that there might be soluble CD58 in goat serum.
Keywords:Goat serum  Free form CD2  Soluble CD58.
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