| 牙鲆胚胎阶段pax6、tbx3和rx1基因的表达 |
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| 引用本文： | 陈洁,鲍宝龙. 牙鲆胚胎阶段pax6、tbx3和rx1基因的表达[J]. 上海海洋大学学报, 2015, 24(3): 321-325 |
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| 作者姓名： | 陈洁 鲍宝龙 |
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| 作者单位： | 上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室, 上海 201306,上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室, 上海 201306 |
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| 基金项目： | 国家自然科学基金(31072201) |
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| 摘 要： |
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| 关 键 词： | 牙鲆 眼睛发育 pax6基因 tbx3基因 rx1基因 眼睛移动 |
| 收稿时间： | 2014-07-15 |
| 修稿时间： | 2015-03-16 |
Expression of pax6, tbx3 and rx1 genes during embryonic development in flounder (Paralichthys olivaceus) |
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| CHEN Jie and BAO Baolong. Expression of pax6, tbx3 and rx1 genes during embryonic development in flounder (Paralichthys olivaceus)[J]. Journal of Shanghai Ocean University, 2015, 24(3): 321-325 |
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| Authors: | CHEN Jie and BAO Baolong |
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| Affiliation: | The Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China and The Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China |
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| Abstract: | During eye development, pax6 and tbx3 define retina field, and rx1 defines optic cup field. We investigated whether pax6, rx1 and tbx3 expressed differently between left and right retinas in Paralichthys olivaceus. Quantity RT-PCR showed that pax6, tbx3and rx1 began to express at 20 hours past fertilization. The whole mount RNA in situ hybridization further showed that pax6 expressed mainly in the left and right retinas, and the expression region of left retina is a little bigger than that in right retina. The signal of tbx3 and rx1 was seen in the retina and their expressional region showed no visible difference between left and right retinas. The difference in expression region of pax6 between left and right retinas reminds us whether there exists a possibility that the difference will cause cell number difference between supraorbital and suborbital areas, which finally causes eye migration through the induction of thyroid hormone during metamorphosis. |
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| Keywords: | Paralichthys olivaceus eye development pax6 rx1 tbx3 eye migration |
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