| 毛竹小RNA高通量测序及病毒分析 |
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| 引用本文： | 范春节,王晖,卢孟柱.毛竹小RNA高通量测序及病毒分析[J].林业科学研究,2014,27(3):335-340. |
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| 作者姓名： | 范春节 王晖 卢孟柱 |
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| 作者单位： | 中国林业科学研究院林业研究所, 林木遗传育种国家重点实验室, 北京 100091;中国林业科学研究院热带林业研究所, 广东 广州 510520;中国林业科学研究院林业研究所, 林木遗传育种国家重点实验室, 北京 100091;NERC/Centre for Ecology and Hydrology (CEH) Oxford, Mansfield Road, Oxford OX1 3SR, UK;中国林业科学研究院林业研究所, 林木遗传育种国家重点实验室, 北京 100091 |
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| 基金项目： | 国家自然科学基金海外及港澳台基金“利用小RNA组研究竹子中的抗病毒免疫及其与竹子开花的关系”（201131028004） |
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| 摘 要： | 以毛竹叶片为材料,采用小RNA高通量测序结合生物信息学对小RNA数据库进行组装,进一步分析了毛竹中存在的病毒和类病毒,并采用RT-PCR和RACE进行验证。结果表明:在竹子样品中存在水稻东格鲁病毒(RTBV),覆盖率达到91.0%。在毛竹样品中扩增得到1 992 bp RTBV病毒类似序列,占其基因组的24.9%。RTBV病毒在多个毛竹样品中存在且不存在多态性。RTBV病毒可能是一个古老的植物病毒,在进化过程中禾本科植物将其序列整合到基因组中来防御RTBV病毒的浸染。
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| 关 键 词： | 毛竹 高通量测序 小RNA RTBV病毒 |
| 收稿时间： | 7/8/2012 12:00:00 AM |
Small RNA Analysis Using High-Throughput Sequencing and Virus Investigation in Bamboo (Phyllostachys edulis) |
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| FAN Chun-jie,WANG Hui and LU Meng-zhu.Small RNA Analysis Using High-Throughput Sequencing and Virus Investigation in Bamboo (Phyllostachys edulis)[J].Forest Research,2014,27(3):335-340. |
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| Authors: | FAN Chun-jie WANG Hui and LU Meng-zhu |
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| Institution: | State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China;Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520, Guangdong, China;State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China;NERC/Centre for Ecology and Hydrology (CEH), Mansfield Road, Oxford OX1 3SR, UK;State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China |
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| Abstract: | The leaves of Phyllostachys edulis were harvested for RNA extraction, a sRNA library was constructed and sequenced by using the Solexa high-throughput sequencing technology. Bioinformatics was performed to identify the sRNAs and viruses. RT-PCR and RACE techniques were explored to identify and confirm the viruses or viroid in bamboo. The assembly of sRNA in all samples revealed that a sequence which covered 91.0% of rice tungro bacilliform virus (RTBV) genome existed widely in bamboo samples. Meanwhile, 1 992bp DNA sequence which covered 24.9% of RTBV completed genome was amplified and no polymorphism was observed in this samples. It concluded that RTBV virus may exist in planta in ancient time and was late integrated into the plant genomes against the RTBV infection. |
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| Keywords: | Phyllostachys edulis high-throughput sequencing small RNA RTBV |
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