| 苹果品种试管苗叶片再生不定芽 |
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| 引用本文： | 吴禄平,张志宏.苹果品种试管苗叶片再生不定芽[J].沈阳农业大学学报,1995,26(2):131-135. |
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| 作者姓名： | 吴禄平 张志宏 |
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| 作者单位： | 沈阳农业大学园艺系 |
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| 摘 要： | 建立了富士和乔纳苹果的试管苗叶片再生系统。在适宜条件下，乔纳金叶片再生频率达１００％，富士叶片则达９０％以上。叶片接种后，立即进行一段时间的暗培养是必需的。培养基附加水解乳蛋白（ＬＨ）２５０ｍｇ／Ｌ，可以显著提高富士叶片再生频率，附加新霉素７５ｍｇ／Ｌ，可以完全抑制芽再生和愈伤组织形成。
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| 关 键 词： | 组织培养 苹果树 品种 试管苗 |
Adventitious Shoot Regeneration from Leaves of in Vitro Growing Shoots of Apple Cultivars |
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| Wu Luping, Zhang Zhihong, Lin Lihua, Sun Xiaorong, Jing Shixi.Adventitious Shoot Regeneration from Leaves of in Vitro Growing Shoots of Apple Cultivars[J].Journal of Shenyang Agricultural University,1995,26(2):131-135. |
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| Authors: | Wu Luping Zhang Zhihong Lin Lihua Sun Xiaorong Jing Shixi |
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| Abstract: | An adventitious shoot regeneration system for apple cvs. Fuji and Jonagold has been established Under optimum conditions, the shoot regeneration frequencies of leavess of Jonagold and Fuji were 100% and more than 90%, respectively. After inoculation,dark incubation was necessary. The shoot regenertation frequency of leaves of Fuji enhanced significantly on medium containing 250mg/L lactalbumin ydrolysate (LH).Adding 75mg/L neomycin to the medium could inhibit shoot regeneration and callus formation totally. |
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| Keywords: | Apple organ incubation |
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