聚丙烯酰胺凝胶柱分离制备高纯度纤维低聚糖的研究

利用聚丙烯酰胺凝胶柱可分离制备纤维低聚糖.在其它分离条件相同、洗脱速度不同的情况下,完成一次分离需要的洗脱剂用量是不变的,洗脱剂用量对低聚糖的分离没有影响.采用一次分离技术可得到纯度分别为96.67%、68.76%和82.00%的纤维五糖、四糖和三糖组分,纤维低聚糖混合物的回收率为81.38%.将多次分离得到的各低聚糖组分在相同的分离条件下进行二次分离,五糖、四糖和三糖的纯度分别提高到98.45%、98.65%和98.12%,回收率分别为90.91%、47.42%和68.68%.

Preparative Separation of High-purity Cellooligosaccharides by Polyacrylamide Gel Column

A procedure for preparative separation of purified cellooligosaccharides was developed by polyacrylamide gel column.Under similar conditions,the dosage of eluent for finishing a one-step separation process did not change with the eluting rate.The dosage of eluent had no effect on the separation.The purities of fractions of cellopentaose,cellotetraose and cellotriose separated by one-step process were 96.67 %,68.76 % and 82.00 %,respectively.The overall recovery of cellooligosaccharides mixture was 81.38 %.High-purity cellooligosaccharides were obtained with purity of 98.45 % for cellopentaose,98.65 % for cellotetraose and 98.12 % for cellotriose by the second separation under the same separating conditions.The corresponding recoveries for cellopentaose,cellotetraose and cellotriose were 90.91 %,47.42 % and 68.68 %,respectively.