| 2个不同类型尖叶胡枝子的微形态和分子标记鉴定 |
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| 引用本文： | 杨秀芳,孙启忠.2个不同类型尖叶胡枝子的微形态和分子标记鉴定[J].中国农业科技导报,2009,11(1):92-95. |
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| 作者姓名： | 杨秀芳 孙启忠 |
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| 作者单位： | （1.甘肃农业大学草业学院, ,兰州 730070； 2.中国农业科学院草原研究所, ,呼和浩特 010010） |
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| 基金项目： | 国家科技支撑计划，国家公益性行业(农业)科研专项基金，农业部引进国际先进农业科学技术计划(948计划) |
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| 摘 要： | 通过扫描电镜观察到叶片浓绿型和叶片黄绿型2个不同类型尖叶胡枝子的豆荚和种皮微形态具有明显差
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| 关 键 词： | 尖叶胡枝子 扫描电镜 ISSR-PCR 微形态 分子标记 |
| 收稿时间： | 2008-04-16 |
| 修稿时间： | 2008-07-08 |
Micromorphology and Molecular Marker Identification of
Two Different Types of Lespedeza hedysaroides |
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| YANG Xiu-fang,SUN Qi-zhong.Micromorphology and Molecular Marker Identification of
Two Different Types of Lespedeza hedysaroides[J].Journal of Agricultural Science and Technology,2009,11(1):92-95. |
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| Authors: | YANG Xiu-fang SUN Qi-zhong |
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| Institution: | (1.College of Grassland Science, Gansu Agricultural University, Lanzhou 730070；;
2. Grassland Research Institute, Chinese Academy of Agricultural Sciences, Hohhot 010010, China） |
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| Abstract: | Two different types of L. hedysaroides with yellow-green leaf or dark-green leaf showed
obvious differences in micromorphological features of pod-coat and seed-coat by scanning
electron microscope (SEM) observation. These micromorphological features can be used for
identification of two different types of L. hedysaroides. ISSR-PCR molecular marker was
also used to identify two different types of L. hedysaroides. Among eight primers for
experiments, the primer UBC-880 was able to distinguish the examined two different types
of L. hedysaroides. L. hedysaroides with dark-green leaf has a polymorphic band in
500~600 bp. ISSR-PCR molecular marker can be used to identify two different types of L.
hedysaroides on DNA level.
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| Keywords: | ISSR-PCR |
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