Ectopic expression of GmPAP3 enhances salt tolerance in rice by alleviating oxidative damage |
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| Authors: | Lixi Deng Fen Chen Liping Jiang Hon‐Ming Lam Guoying Xiao |
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| Institution: | 1. Key Laboratory of Agro‐ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, , Changsha, 410125 China;2. University of the Chinese Academy of Sciences, , Beijing, 100049 China;3. Center for Soybean Research of the State Key Laboratory of Agrobiotechnology and School of Life Sciences, The Chinese University of Hong Kong, , Hong Kong SAR, China |
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| Abstract: | GmPAP3, a purple acid phosphatase from soybean (Glycine max), was previously shown to alleviate salt stress in BY‐2 cells and Arabidopsis thaliana by reducing oxidative damage. To make use of GmPAP3 for crop improvement, we investigated whether the protective function of GmPAP3 is persistent in rice. Compared with the untransformed wild type, the transgenic rice plants exhibited enhanced germination rate, longer shoots and roots, and higher survival rate under salt stress, when compared to the untransformed control. In addition, the transgenic plants also showed increased activity of superoxide dismutase (SOD) and catalase (CAT), proline content, water content and chlorophyll content, but decreased electrolyte leakage and malondialdehyde content in leaves. These results indicate that the expression of GmPAP3 could be used to enhance salt tolerance in rice. |
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| Keywords: | purple acid phosphatase rice salt tolerance transgenic plants |
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