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| 茶树紫色芽叶中花青素的提取-层析分离及初步鉴定 | |
| 引用本文： | 李双伶,郭俊凌,杜晓. 茶树紫色芽叶中花青素的提取-层析分离及初步鉴定[J]. 安徽农业科学, 2009, 37(28) |
| 作者姓名： | 李双伶 郭俊凌 杜晓 |
| 作者单位： | 1. 四川省茶业科学与工程重点实验室,四川雅安,625014 2. 四川农业大学园艺学院,四川雅安,625014 3. 四川省茶业科学与工程重点实验室,四川雅安,625014;四川农业大学园艺学院,四川雅安,625014 |
| 基金项目： | 四川省教育厅重点实验室项目，四川农业大学青年科技创新基金项目 |
| 摘 要： | [目的]为茶叶中花青素的提取、分离与鉴定提供方法支持。[方法]以茶树紫色芽叶为原料,通过提取、除杂获得花青素粗提物,再通过柱层析和薄层层析分离获得一种花青素,采用紫外-可见光谱法对其结构进行鉴定。[结果]花青素粗提物在465～550 nm波长范围内有花青素特有的吸收峰,可以初步确定花青素粗提物中含有花青素。花青素粗提物在浓盐酸中显粉红色,且其在KI·I_2试剂的作用下不产生棕色沉淀,表明花青素主要存在于花青素粗提物中,而且其中不含有咖啡碱。显色鉴定结果表明,柱层析出的F8含有花青素。显色和紫外-可见光谱法的鉴定结果表明,薄层层析出的C2为花青素组分。以紫芽茶为原料,采用乙醇水溶液提取花青素,并通过石油醚脱脂,三氯甲烷、乙酸乙酯除杂获得花青素粗提物,所得花青素产率为6.44%。[结论]该研究对茶树资源的开发利用具有重要意义。 |
| 关 键 词： | 花青素 层析分离 紫外-可见光谱法 结构鉴定 |
Extraction and Chromatographic Separation of Anthocyanin from Purple Bud and Leaves of Tea Tree and Its Primary Identification |
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| LI Shuang-ling et al. Extraction and Chromatographic Separation of Anthocyanin from Purple Bud and Leaves of Tea Tree and Its Primary Identification[J]. Journal of Anhui Agricultural Sciences, 2009, 37(28) | |
| Authors: | LI Shuang-ling et al |
| Affiliation: | LI Shuang-ling et al(Key Laboratory for Tea Science , Engineering of Sichuan Province,Yaan,Sichuan 625014) |
| Abstract: | [Objective]The aim of the research was to provide methodological support for the extraction,separation and identification of anthocyanin from tea.[Method]With the purple bud and leaves of tea tree as raw materials,the crude extracts of anthocyanin were obtained through extraction and removing impurity.Then a kind of enthocyanin was obtained through separation with column chromatography and TLC and its structure was identified by UV-visual spectrometry.[Result]The crude extracts of anthocyanin had the peculi... |
| Keywords: | Anthocyanin Chromatographic separation UV-visual spectrometry Structural identification |
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