酵母菌0732-1产生的抑细菌物质特性研究

<正>哈密瓜细菌性果斑病是近年来暴发流行的一种瓜类病害,也是我国对内对外检疫性重大植物病害。该病由燕麦嗜酸菌西瓜亚种(Acidovorax avenae subsp.citrulli,Aac)引起,具有发病快、危害广、损失重、防治难的特点,一旦发生往往给瓜产区造成严重经济损失。据报道1],2007年新疆石河子市温室栽培的西瓜叶片上曾获得一株对Aac有

Characteristics of antibacterial substance produced by the yeast strain 0732-1

Bioactive metabolites produced by Pichia anomala 0732-1 displayed significant antibacterial activity to Acidovorax avenae subsp. citrulli (Aac) which caused bacterial fruit blotch. The antibiotic capacity, antibacterial activity and stability of crude antagonistic extract of P. anomala 0732-1 were tested and the active substances were isolated. The results showed that the antibacterial material was not sensitive to ultraviolet radiation and temperature, and it was stable at pH<9. The antibacterial material was purified by precipitation with (NH₄)₂SO₄ (10%-100%, w/v), extraction with benzene, acetone, chloroform, phenixin, ethanol and ethyl acetate, and distillation method. However, it could not be purified by precipitation with (NH₄)₂SO₄ or by extraction with organic solvent. It could be partially distilled out by distillation. In addition, antibacterial substances produced by P. anomala 0732-1 had significant inhibitory effect against Hami melon seed infested by Aac (bacterial suspension 1×10⁸ CFU/mL). When infected seeds were treated with crude antagonistic extract of P. anomala 0732-1 for 120 min, the seeding disease index was 1.67 during the cotyledon. This study suggested that P. anomala 0732-1 had the potential to be developed as an antagonistic agent for control Hami bacterial fruit blotch.