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| 火龙果细菌性软腐病菌的分离与鉴定 | |
| 引用本文： | 林武镇,廖富荣,陈细红,陈青,陈红运,黄蓬英,林石明. 火龙果细菌性软腐病菌的分离与鉴定[J]. 植物病理学报, 2015, 45(2): 220-224 |
| 作者姓名： | 林武镇 廖富荣 陈细红 陈青 陈红运 黄蓬英 林石明 |
| 作者单位： | 厦门出入境检验检疫局检验检疫技术中心,厦门 361026; 福建农林大学 生物农药与化学生物学教育部重点实验室,福州 350002 |
| 基金项目： | 质检公益性行业科研专项项目(201410076);福建省自然科学基金资助项目(2011J01242);福建出入境检验检疫局科技项目(FK2011-07) |
| 摘 要： | <正>火龙果(Hylocereus undatus Britt.et Rose)属仙人掌科三角柱属植物,是集水果、花卉、蔬菜、保健为一体的热带亚热带果树,在中美洲、美国南部地区,以及以色列、越南、泰国和我国的台湾、海南、广东、福建等地均有种植。近年来,随着火龙果种植面积不断扩大,火龙果病害呈逐渐加重趋势,严重威胁火龙果的产业发展。火龙果细菌性病害主要由欧文氏菌(Erwinia sp.)、阴沟肠杆菌(En- |
| 关 键 词： | Enterobacter species Hylocereus undatus soft rot |
| 收稿时间： | 2013-12-27 |
Isolation and identification of the pathogen causing soft rot in Hylocereus undatus |
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| LIN Wu-zhen,LIAO Fu-rong,CHEN Xi-hong,CHEN Qing,CHEN Hong-yun,HUANG Peng-ying,LIN Shi-ming. Isolation and identification of the pathogen causing soft rot in Hylocereus undatus[J]. Acta Phytopathologica Sinica, 2015, 45(2): 220-224 | |
| Authors: | LIN Wu-zhen LIAO Fu-rong CHEN Xi-hong CHEN Qing CHEN Hong-yun HUANG Peng-ying LIN Shi-ming |
| Affiliation: | Inspection and Quarantine Technology Center, Xiamen Entry-Exit Inspection and Quarantine Bureau, Xiamen 361026, China; Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agricultural and Forestry University, Fuzhou 350002, China |
| Abstract: | A bacteria strain 5877-A1 was isolated from dragon fruit (Hylocereus undatus) with typical soft rot symptom imported from Taiwan, China. Colonies of the isolate on nutrient agar medium were circular, oyster white, protuberant, smooth and mucous. The bacteria were Gram-negative and short rod-shaped in (0.44-0.88) μm×(0.83-2.82) μm. Inoculation of bacterial isolates into dragon fruit seedlings yielded characteristic soft rot symptom. Then it was identified as Enterobacter cloacea by Biolog Gen III and MALDI-TOF-MS test. Sequence analysis of 16S rRNA gene showed that it shared 99.9% sequence homology with E. asburiae, E. cancerogenus and E. hormaechei. Sequence and phylogenetic analysis of gyrB gene indicated that it was closely related to, but distinguishable from, E. asburiae and E. mori. The results showed it maybe an unreported species of the genus Enterobacter. This is the first report of Enterobacter sp. in dragon fruit from Taiwan. |
| Keywords: | Enterobacter Hylocereus undatus soft rot |
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