| 水稻瘤矮病毒 P3、P7、P8、Pn9、Pn10、Pn11、Pn12 的酵母双杂交载体的构建及自激活效应检测 |
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| 引用本文： | 吴建国,蔡丽君,胡梅群,谢荔岩,林奇英,吴祖建,谢联辉.水稻瘤矮病毒 P3、P7、P8、Pn9、Pn10、Pn11、Pn12 的酵母双杂交载体的构建及自激活效应检测[J].热带作物学报,2009,30(9):1364-1368. |
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| 作者姓名： | 吴建国 蔡丽君 胡梅群 谢荔岩 林奇英 吴祖建 谢联辉 |
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| 作者单位： | 福建农林大学植物病毒研究所,福建省植物病毒学重点实验室,福建福州,350002 |
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| 基金项目： | 农业部农业公益性行业科研专项，教育部博士点专项科研基金，福建省科技厅项目 |
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| 摘 要： | 将编码水稻瘤矮病毒P3、P7、P8、Pn9、Pn10、Pn11、Pn12的S3,S7,S8,S9,S10,S11和S12基因分别克隆到酵母表达载体pGBKT7和pGADT7中,转化AH109酵母细胞,结果显示:除pGBK-S10转化子可以在SD/-Trp和SD/-His营养缺陷固体培养基上正常生长外,其它转化子均只能在SD/-Trp或SD/-Leu营养缺陷固体培养基上正常生长.通过α-半乳糖苷酶活性分析揭示,除pGBK-S10可以在SD/-Trp/X-α-Gal平板上生长并变蓝外,其它均不变蓝.结果证实:RGDV Pn10在酵母细胞中具有转录激活活性,融合GAL4的RGDV Pn10蛋白可以在酵母细胞内激活HIS3和MEL1报告基因的表达.暗示该蛋白可能在病毒的侵染和复制过程中参与调控病毒或寄主基因的转录表达.
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| 关 键 词： | 水稻瘤矮病毒 转录激活活性 酵母表达载体 |
Construction of Yeast Two-hybrid Vectors of P3, P7, P8, Pn9, Pn10, Pn11 and Pn12 of Rice Gall Dwarf Virus and Identification of Their Self-activation |
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| Wu Jianguo,Cai Lijun,Hu Meiqun,Xie Liyan,Lin Qiying,Wu Zujian and Xie Lianhui.Construction of Yeast Two-hybrid Vectors of P3, P7, P8, Pn9, Pn10, Pn11 and Pn12 of Rice Gall Dwarf Virus and Identification of Their Self-activation[J].Chinese Journal of Tropical Crops,2009,30(9):1364-1368. |
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| Authors: | Wu Jianguo Cai Lijun Hu Meiqun Xie Liyan Lin Qiying Wu Zujian and Xie Lianhui |
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| Institution: | Institute of Plant Virology, Fujian Agriculture and Forestry University/Fujian Key Laboratory for Plant Virology;Institute of Plant Virology, Fujian Agriculture and Forestry University/Fujian Key Laboratory for Plant Virology;Institute of Plant Virology, Fujian Agriculture and Forestry University/Fujian Key Laboratory for Plant Virology;Institute of Plant Virology, Fujian Agriculture and Forestry University/Fujian Key Laboratory for Plant Virology;Institute of Plant Virology, Fujian Agriculture and Forestry University/Fujian Key Laboratory for Plant Virology;Institute of Plant Virology, Fujian Agriculture and Forestry University/Fujian Key Laboratory for Plant Virology;Institute of Plant Virology, Fujian Agriculture and Forestry University/Fujian Key Laboratory for Plant Virology |
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