| 辣椒叶片基因组DNA提取方法的研究 |
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| 引用本文： | 周春阳,谢立波,李景富,周宇.辣椒叶片基因组DNA提取方法的研究[J].辣椒杂志,2011(3):35-37. |
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| 作者姓名： | 周春阳 谢立波 李景富 周宇 |
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| 作者单位： | 1. 东北农业大学园艺学院,哈尔滨,150030
2. 黑龙江省农科院园艺分院,哈尔滨,150069 |
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| 基金项目： | 航天育种工程项目(2009HT2000); 黑龙江省自然基金重点项目(ZD200818-01),黑龙江省自然基金项目(C200943); 国家大宗蔬菜产业技术体系哈尔滨综合试验站项目(CARS-25-G-11) |
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| 摘 要： | 本项研究采用SDS法、CTAB1法、CTAB2法及CTAB3法对辣椒叶片基因组DNA进行提取，通过琼脂糖凝胶电泳法与紫外吸收检测法对DNA的纯度进行检测。试验证明几种提取方法均能提取出辣椒叶片的基因组DNA，其中CTAB3法提取的DNA经琼脂糖凝胶电泳检测得到一条迁移率很低的整齐清晰的DNA条带，所提取DNA的质量和纯...
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| 关 键 词： | 辣椒 DNA提取 琼脂糖凝胶电泳检测 紫外吸收检测 |
The Extraction of Genomic DNA from Hot Pepper |
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| Zhou Chunyang,Xie Libo,Li Jingfu,Zhou Yu.The Extraction of Genomic DNA from Hot Pepper[J].Journal of China Capsicum,2011(3):35-37. |
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| Authors: | Zhou Chunyang Xie Libo Li Jingfu Zhou Yu |
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| Institution: | Zhou Chunyang 1 Xie Libo 2 Li Jingfu 1 Zhou Yu 2 (1 College of Horticulture,Northeast Agricultural University,Harbin 150030 ) (2 Horticultural Sub-Academy,Heilongjiang Academy of Agricultural Sciences,Harbin 150069 ) |
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| Abstract: | In this experiment, SDS,CTAB 1,CTAB 2 and CTAB 3 methods were used to extract DNA from hot pepper leaves, and agarose gel electrophoresis and UV-spectrophotometry were done to test DNA purity. The results indicated that all the methods could extract DNA from hot pepper leaves; the DNA extracted with the CTAB 3 method showed a consistent and clear DNA band of very low migration rate in the agarose gel electrophoresis, had higher quality and purity, and got the typical absorption spectra in the UV-spectrophot... |
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| Keywords: | hot pepper DNA extraction agarose gel electrophoresis detection UV-spectrophotometry detection |
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