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| 氯粘康酸环异构酶基因克隆及序列研究 | |
| 引用本文： | 钟文辉,何国庆,孙 明,喻子牛,冯孝善.氯粘康酸环异构酶基因克隆及序列研究[J].中国生态农业学报,2005,13(3):112-115. |
| 作者姓名： | 钟文辉 何国庆 孙 明 喻子牛 冯孝善 |
| 作者单位： | 1. 南京师范大学化学与环境科学学院,南京,210097 2. 浙江大学生物系统工程与食品科学学院,杭州,310029 3. 华中农业大学生命科学技术学院,武汉,430070 4. 浙江大学环境与资源学院,杭州,310029 |
| 基金项目： | 国家自然科学基金项目(30080013)，农业微生物学国家重点实验室开放基金项目(AML0210)资助 |
| 摘 要： | 研究从土壤中分离出1株降解2,4-二氯酚能力较强的假单胞菌菌株“GT241-1”,并从中克隆出参与降解2,4-二氯酚的氯粘康酸环异构酶基因(dcpC),该基因编码的氯粘康酸环异构酶可将2,4-二氯-顺,顺-粘康酸转化为反式-2-氯-双烯内酯。采用的基因克隆策略是用Southern杂交对其邻近基因进行定位后构建基因组文库,再用斑点杂交从基因文库中筛选目的转化子。经序列测定得知dcpC基因编码区1110bp,且核苷酸和推测的编码氨基酸序列分析表明dcpC属氯粘康酸环异构酶基因家族,并与该基因家族的其他基因有一定差异。 |
| 关 键 词： | 假单胞菌 2 4-二氯酚 氯粘康酸环异构酶基因 基因克隆 |
| 收稿时间： | 2004/9/10 0:00:00 |
| 修稿时间： | 2004年9月10日 |
Cloning and sequence analysis of chloromuconate cycloisomerase gene |
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| ZHONG Wen-Hui,HE Guo-Qing,SUN Ming,YU Zi-Niu and FENG Xiao -Shan.Cloning and sequence analysis of chloromuconate cycloisomerase gene[J].Chinese Journal of Eco-Agriculture,2005,13(3):112-115. | |
| Authors: | ZHONG Wen-Hui HE Guo-Qing SUN Ming YU Zi-Niu and FENG Xiao -Shan |
| Institution: | College of Chemistry and Environmental Science, Nanjing Normal University,Nanjing 210097,China;College of Biosystem Engineering and Food Sciences, Zhejiang University, Hangzhou 310029,China;College of Life Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;College of Life Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;College of Environmental and Resource Sciences,Zhejiang University,Hangzhou 310029,China |
| Abstract: | A 2,4-dichlorophenol degrading Pseudomonas strain GT241-1 was isolated from a soil sample. The chloromuconate cycloisomerase gene, designated as dcpC which encodes chloromuconate cycloisomerase involved in transforming 2,4-dichloro-cis,cis-muconate into trans-2-chlorodienelactone,was cloned from this bacterium strain.The gene cloning strategy was to construct genomic library after location of its neighbouring gene by Southern blot and to screen the aim transformant by dot blotting.Sequencing results show that the length of dcpC is 1110bp.The sequence analysis of dcpC and the deduced amino acid show that dcpC belongs to chloromuconate cycloisomerase gene family and is different from other genes in the gene family. |
| Keywords: | Pseudomonas 2 4-dichlorophenol Chloromuconate cycloisomerase gene Gene cloning |
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