Application of bovine microsatellite markers for genetic diversity analysis of European bison (Bison bonasus) |
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| Authors: | T. Roth,I. Pfeiffer,K. Weising,& B. Brenig |
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| Affiliation: | Institute of Veterinary Medicine, Georg-August-University of Göttingen, Göttingen, Germany; Institute of Biology, University of Kassel, Kassel, Germany |
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| Abstract: | In this study, the cross‐amplification of a commercial multiplex set of 11 cattle (Bos taurus) microsatellites was tested on a panel of 35 European bison (Bison bonasus) individuals. After polymerase chain reaction optimization, all loci cross‐amplified successfully in investigated bisons. Number of alleles and observed and expected heterozygosity per locus are in the range of 2–4, 0.086–0.629 and 0.288–0.621 respectively. The availability of a heterologous set of multiplexed microsatellite markers derived from cattle opens an avenue for collecting profound genetic data for efficient conservation management strategies of the European bison. |
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| Keywords: | Bison bonasus conservation management European bison microsatellite markers multiplex PCR |
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