四倍体刺槐离体培养及其不定根发育和叶片解剖观察

 .通过茎段离体培养建立了四倍体刺槐无性系的微体繁殖体系。结果表明:基本培养基为MS或WPM培养基。BA、NAA影响芽的增殖生长,在一定的范围内,BA对芽的增殖影响比NAA大,而NAA对芽的高生长影响比BA大,二者的比例对芽的增殖生长也有影响,芽增殖生长的最适BA和NAA组合应为BA0.5mg·L-1+NAA0.1mg·L-1。四倍体刺槐无性系生根的最适生长调节物质配比为NAA0.25mg·L-1+IBA0.4mg·L-1。对试管苗不定根发育过程及分步炼苗叶片结构的变化进行了解剖观察,发现试管苗嫩梢无潜伏根原基,不定根由诱生根原基发育形成,诱生根原基源于髓射线细胞的分裂和分化,植株叶解剖观察,进一步证明了分步炼苗可提高移栽成活率。

Study on Tissue Culture of Tetraploid Black Locus Clone and Anatomical Observation on the Development of Adventitious Roots and Structure of Leaves

A micro-propagation system for tetraploid black locust ( Robinia pseudoacacia ) was established by using its shoots. The optimum medium for shoot multiplication and growth was MS + BA0.5 mg·L^-1 + NAA0. 1 mg·L^-1 . The best medium for rooting was 1/2 MS + IBA 0.4 mg·L^-1 + NAA0.25 mg·L^-1. The adventitious root development of shoots in test tube was also observed by means of the anatomical method. Latent root primordium was not found in shoots in test tubes. The induced root primordium which originated from the division and differentiation of pith ray cell developed into the adventitious roots. It had been proved that survival percentage had been increased by means of the plantlets acclimated through leaf anatomical observation.