| 花青素合成关键酶基因的定位及结构分析 |
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| 引用本文： | 朱晓双,刘春燕,杨振,蒋洪蔚,陈庆山,胡国华.花青素合成关键酶基因的定位及结构分析[J].大豆科学,2011,30(1). |
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| 作者姓名： | 朱晓双 刘春燕 杨振 蒋洪蔚 陈庆山 胡国华 |
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| 作者单位： | 朱晓双,杨振,陈庆山,ZHU Xiao-shuang,YANG Zhen,CHEN Qing-shan(东北农业大学,农学院,黑龙江,哈尔滨,150030);刘春燕,蒋洪蔚,LIU Chun-yan,JIANG Hong-wei(黑龙江省农垦科研育种中心,黑龙江,哈尔滨,150090);胡国华,HU Guo-hua(黑龙江省农垦科研育种中心,黑龙江,哈尔滨,150090;国家大豆工程技术研究中心,黑龙江,哈尔滨,150050) |
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| 摘 要： | 为研究大豆花青素合成途径关键酶的基因标记和结构信息,利用大豆基因组和染色体标记数据,对16个花青素合成关键酶基因(苯丙氨酸解氨酶(PAL)、查尔酮合酶(CHS,包括9个成员)、黄烷酮-3-羟化酶(F3H)、苯基苯乙烯酮黄烷酮异构酶(CHI)、二氢黄酮醇还原酶(DFR)和4-香豆酰CoA连接酶(4CL),进行基因遗传图和物理图定位和基因结构分析.结果表明:16个基因分别定位在A1、A2、B1、B2、D1a、D1b、D2、I、K、O等10个连锁群上,并获得了基因所在序列两侧标记.利用大豆的cDNA和gDNA序列信息,获得了16个基因的结构,外显子数目1～7个,内含子数目0～6个,其中PAL、DFR2、GmCHS7是单外显子基因,4CL、CHI、F3H、GmCHS1、GmCHS5、GmCHS8有1个内含子,DFR1、GmCHS2、GmCHS3、GmCHS6有2个内含子,GmCHS4、GmCHS9有3个内含子,GmIRCHS则有6个内含子.
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| 关 键 词： | 大豆 花青素 基因 定位 基因结构 |
Mapping and Structure Analysis of Key Enzyme Genes in Anthocyanin Synthesis of Soybean |
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| ZHU Xiao-shuang,LIU Chun-yan,YANG Zhen,JIANG Hong-wei,CHEN Qing-shan,HU Guo-hua.Mapping and Structure Analysis of Key Enzyme Genes in Anthocyanin Synthesis of Soybean[J].Soybean Science,2011,30(1). |
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| Authors: | ZHU Xiao-shuang LIU Chun-yan YANG Zhen JIANG Hong-wei CHEN Qing-shan HU Guo-hua |
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| Institution: | ZHU Xiao-shuang1,LIU Chun-yan2,YANG Zhen1,JIANG Hong-wei2,CHEN Qing-shan1,HU Guo-hua2,3(1.College of Agriculture,Northeast Agricultural University,Harbin 150030,2.The Crop Research and Breeding Center of Land-Reclamation,Harbin 150090,3.The National Research Center of Soybean Engineering and Technology,Harbin 150050,Heilongjiang,China) |
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| Abstract: | Seedcoat mottling would be induced in soybean when infected by soybean mosaic virus,which could be greatly decrease the economic value.Seedcoat mottling of soybean was due to the abnormal accumulation of flavonoid.And anthocyanin belongs to flavonoid was a kind of major secondary metabolites.Sixteen genes of key enzyme in anthocyanin synthesis,including PAL,chalcone synthase(9 members of CHS family),F3H,CHI,DFR,and 4CL,were mapped on the soybean linkage groups,based on the soybean genome sequence and marker... |
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| Keywords: | Soybean Anthocyanin Gene Mapping Gene structure |
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