高温下清凉冲剂对鸡免疫器官中CD4+、CD8+ T细胞数量的影响

采用免疫组化方法检测鸡免疫器官中CD4^＋、CD8^＋T细胞数量，探讨高温下清凉冲剂对机体细胞免疫机能的影响及其药理作用。108只农大3号35目龄公雏，随机分为3组，每纽36只鸡，即常温对照组、高温对照组和高温用药组。用药组饲喂中药煎荆（浓度为1g／ml），用药量与饲料量比为1：1000。在试验的第1、4、8、10天各组捕杀5只。分别采取胸腺、脾脏、法氏囊检测。结果高温下。用药组免疫器官中CD4^＋、CD8^＋T细胞数量均高于高温对照组。第8、10天差异极显著（P〈0．01）。表明高温下清凉冲剂可以增加鸡免疫器官中CD4^＋、CD8^＋T细胞数量，有效地提高鸡细胞免疫水平。

Effect of Qingliang electuary on the amounts of CD4+ and CD8+ T - cells under high temperature in chickens

Objective: To study the effects of Qingliang electuary on the amounts of CD4~+ and CD8~+ T-cells under high temperature in chickens. Methods: 108 Nongda III 35-day-old male chickens were randomly divided into two control groups respectively under normal and high temperature, and treatment group under high temperature, which was given Qingliang electuary (0.1%) in water. Thymus, spleen, and bursa of Fabricius were collected from 5 chickens each group on 1~ st , 4~ th , 8~ th and 10~ th , respectively, to determine the average positive rates of CD4~+ and CD8~+ T-cells in the immune organs of chicken by immunohistochemistry and motic image analyzing. Results: The amounts of CD4~+ and CD8~+ T-cells in the treatment group were higher than that of the control (P<0.05 or P<0.01). Conclusion: Qingliang electuary could increase the amounts of CD4~+ and CD8~+ T-cells and improve the cellular immune function under high temperature.