Abstract: | The changes in the ultrastructure of gonadotrops and in the plasma levels of LH were studied three, five, seven, ten, twenty and forty days after castration in adult male mice. Gonadotrops in sham operated animals contained numerous granules and moderately developed synthetic organelles. Castration induced mobilization of the secretory granules at three days, and degranulation of gonadotrops and a hypertrophy of the synthetic organelles at seven days. By ten days after castration gonadotrops were greatly enlarged, while in addition at forty days they frequently contained large cytoplasmic vesicles filled with lipid-like material. These changes were paralleled by an initial three fold increase in serum LH three days after castration, and a further ten fold increase in the levels of the hormone forty days after castration. In conclusion, the castration-induced morphologic changes in gonadotrops correlate well with increased release of LH. The subtle differences in the control of timing of LH secretion in mice and rats are discussed. A trans-cytoplasmic route for rapid transport of gonadotropic hormones in the mouse is postulated, and the significance of cytoplasmic vesicles in the castrate discussed. |