| 家蚕HSP70基因的电子克隆与序列分析 |
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| 引用本文： | 房守敏,伍春莲.家蚕HSP70基因的电子克隆与序列分析[J].蚕学通讯,2011,31(1):9-14. |
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| 作者姓名： | 房守敏 伍春莲 |
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| 作者单位： | 西华师范大学生命科学学院; |
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| 基金项目： | 四川省教育厅青年基金(编号:10ZC124) |
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| 摘 要： | 热休克蛋白70(HSP70)广泛分布于生物体中,在蛋白转运、耐热性、细胞保护等方面起着重要作用.本研究以黑腹果蝇的一个HSP70氨基酸序列与家蚕基因组预测的基因库进行tBlastn检索,共获得了多个可能的家蚕HSP70基因,我们对编号为BGIBMGA002381进行了电子克隆.BGIBMGA002381基因的编码区序列...
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| 关 键 词： | 家蚕 热休克蛋白70 电子克隆 序列分析 |
Electronic Cloning and Sequence Analysis of HSP70 Gene in the Silkworm |
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| FANG Shou-min,WU Chun-lian.Electronic Cloning and Sequence Analysis of HSP70 Gene in the Silkworm[J].Newsletter of Sericultural Science,2011,31(1):9-14. |
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| Authors: | FANG Shou-min WU Chun-lian |
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| Institution: | FANG Shou-min,WU Chun-lian(College of Life Science,China West Normal University,Nanchong,Sichuan 637002,China) |
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| Abstract: | Heat shock protein 70(HSP) is widely distributed in organisms and plays important roles in protein transport,heat resistance and cell protection.In this study,an HSP70 amino acid sequence of Drosophila melanogaster was used to do Blastp search in the silkworm genome.Several putative HSP70 genes were found.We chose one of the gene(accession number: BGIBMGA007950) for electronic cloning.The putative coding sequence of BGIBMGA002381 was used to do Blastn search in the silkworm expressed sequence tags(ESTs) dat... |
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| Keywords: | Bombyx mori heat shock protein 70 electronic cloning sequence analysis |
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