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一株来自东祁连山高寒草地土壤纤维素分解真菌培养特性的研究
引用本文:芦光新,刘雯,卞静,傅美涛,陈秀蓉.一株来自东祁连山高寒草地土壤纤维素分解真菌培养特性的研究[J].草原与草坪,2011,31(3):50-55.
作者姓名:芦光新  刘雯  卞静  傅美涛  陈秀蓉
作者单位:1. 甘肃农业大学草业学院/草业生态系统教育部重点实验室/中-美草地畜牧业可持续发展研究中心/甘肃省草业工程实验室,甘肃兰州730070;青海大学农牧学院草业科学系,青海西宁810016
2. 甘肃农业大学草业学院/草业生态系统教育部重点实验室/中-美草地畜牧业可持续发展研究中心/甘肃省草业工程实验室,甘肃兰州,730070
基金项目:国家自然科学基金项目(30471232)资助
摘    要:对一株来自东祁连山高寒草地土壤具有纤维素分解能力的真菌的培养特性进行了研究.结果表明:供试菌株的菌丝生长温度5~35℃,最适生长温度30℃;在参试的pH范围均能生长,并且在pH7.08的环境生长最快;供试菌株具有一定的耐盐和抗干旱胁迫的能力;对不同的碳、氮源利用能力不同,在供试的11种碳源和12种氮源中,乳糖和大豆蛋白...

关 键 词:高寒草地  纤维素分解真菌  培养特性

Study on cultural characteristic of one cellulose-decomposing stains of fungus from alpine grassland soil in eastern Qilian Mountains
LU Gung-xin,LIU Wen,BIAN Jing,FU Mei-tao,CHENG Xiu-rong.Study on cultural characteristic of one cellulose-decomposing stains of fungus from alpine grassland soil in eastern Qilian Mountains[J].Grassland and Turf,2011,31(3):50-55.
Authors:LU Gung-xin  LIU Wen  BIAN Jing  FU Mei-tao  CHENG Xiu-rong
Institution:LU Gung-xin,LIU Wen,BIAN Jing,FU Mei-tao,CHENG Xiu-rong(1.College of Pratacultural Science,Gansu Agricultural University/Key Laboratory of Grassland Ecosystem,Ministry of Education/Sino-U.S.Centers for Grazingland Ecosystem Sustainability/Pratacultural Engineering Laboratory of Gansu Province,Lanzhou 730030,China,2.College of Agro-Pastoral,Qinghai University,Xining,810016,China)
Abstract:Cultural characteristic of one cellulose-decomposing stain of fungus from alpine grassland soil in eastern Qilian Mountains were studied.The results showed that this strains was able to grow at 5~35 ℃.The optimal cultural temperature and pH values for mycelial growth was 30 ℃ and 7.08 respectively.The strain has ability of salt and PEG stress tolerance.It varied in its viability on media containing different carbon and nitrogen nutrients.The preferential carbon and nitrogen source was lactose and soybean peptone,respectively.Compare to the 1.5% water agar medium,the growth of the stain was faster in ryegrass extract,but slower in clover extract.The growth of the mycelium was not significantly difference in medium with different natural cellulose extract.
Keywords:alpine grassland  cellulose-decomposing strains of fungus  cultural characteristic  
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