| 星油藤根腐病的症状和病原鉴定 |
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| 引用本文： | 魏玉倩, 唐婕, 普晓兰, 伍建榕, 童亚丽, 杨植芳. 星油藤根腐病的症状和病原鉴定[J]. 西南林业大学学报, 2018, 38(2): 190-193.doi:10.11929/j.issn.2095-1914.2018.02.030 |
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| 作者姓名： | 魏玉倩 唐婕 普晓兰 伍建榕 童亚丽 杨植芳 |
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| 作者单位： | 1. 西南林业大学国家林业局西南地区生物多样性保育重点实验室，云南 昆明 650224;2. 西南林业大学林学院，云南省高校森林灾害预警控制重点实验室，云南 昆明 650224;3. 云南云投生态环境科技股份有限公司，云南 昆明 650217;4. 西双版纳印奇生物资源开发有限公司，云南 西双版纳 666100 |
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| 基金项目： |
摘要:采用切片和组织分离法对星油藤根腐病病原菌进行分离，结合形态学观察与分子生物学方法对病原菌进行鉴定，按柯赫氏法则对其致病性进行测定。结果表明：PCR技术扩增病菌rDNA-ITS基因，获得长度为550~650 bp的DNA片段；菌株序列与尖孢镰刀菌的序列同源性达到99%；星油藤根腐病的病原菌为尖孢镰刀菌(Fusarium oxysporum)，通过重分离获得该菌株。 |
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| 摘 要： | 采用切片和组织分离法对星油藤根腐病病原菌进行分离，结合形态学观察与分子生物学方法对病原菌进行鉴定，按柯赫氏法则对其致病性进行测定。结果表明：PCR技术扩增病菌rDNA-ITS基因，获得长度为550~650 bp的DNA片段；菌株序列与尖孢镰刀菌的序列同源性达到99%；星油藤根腐病的病原菌为尖孢镰刀菌(Fusarium oxysporum)，通过重分离获得该菌株。
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| 关 键 词： | 星油藤根腐病 症状 病原 鉴定 rDNA-ITS 病害 |
| 收稿时间： | 2017-05-27 |
Identification of Symptoms and Pathogens of Plukenetia volubilis Root Rot |
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| Yuqian Wei, Jie Tang, Xiaolan Pu, Jianrong Wu, Yali Tong and Zhifang Yang. Identification of Symptoms and Pathogens of Plukenetia volubilis Root Rot[J]. Journal of Southwest Forestry University, 2018, 38(2): 190-193.doi:10.11929/j.issn.2095-1914.2018.02.030 |
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| Authors: | Yuqian Wei Jie Tang Xiaolan Pu Jianrong Wu Yali Tong Zhifang Yang |
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| Affiliation: | 1. Key Laboratory of State Forestry Administration for Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming Yunnan 650224, China;2. Key Laboratory of Forest Disaster Warning and Control in Yunnan Higher Education Institutions, College of Forestry, Southwest Forestry University, Kunming Yunnan 650224, China;3. Yunnan Investment Ecology and Environment Technology Co., Ltd., Kunming Yunnan 650217, China;4. Yingqi Biological Resources Development Co., Ltd., Xishuang Banna Yunnan 666100, China |
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| Abstract: | The pathogen of Plukenetia volubilis root rot was isolated using section-cutting and tissue isolation method, identified by morphological observation and molecular biology method, and determined according to the Koch′s rule. The result showed that rDNA-ITS gene was amplified by PCR technique, DNA fragment with a length of 550-650 bp was obtained. The sequence homology between the stain and Fusariun oxysporum was 99%. The pathogen of P.volubilis root rot was F.oxysporum. |
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| Keywords: | Plukenetia volubilis root rot symptom pathogen identification rDNA-ITS disease |
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