| 南方鲇血清凝集素的研究 |
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| 引用本文： | 张黎,袁媛,王娟,顾继锐,吴江,谢显连,徐恒. 南方鲇血清凝集素的研究[J]. 淡水渔业, 2006, 36(6): 29-32 |
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| 作者姓名： | 张黎 袁媛 王娟 顾继锐 吴江 谢显连 徐恒 |
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| 作者单位： | 四川大学生物资源与生态环境教育部重点实验室,成都,610064;通威股份通威水产科技有限公司,成都,610081;四川大学生物资源与生态环境教育部重点实验室,成都,610064;通威股份通威水产科技有限公司,成都,610081 |
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| 基金项目： | 四川省科技攻关项目，通威股份公司科技发展基金 |
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| 摘 要： | 从南方鲇(Silurusmeridionalis)血清分离到一种天然的凝集素分子,能够凝集多种不同的抗原物质,其组成成分为糖蛋白,具有耐热和耐酸碱性,对β-巯基乙醇敏感。凝集反应受温度、离子强度以及时间等因素的影响。经糖的专一性抑制实验显示,蔗糖和半乳糖对凝集反应有抑制作用,说明这2种糖分子是凝集素在识别和凝集过程中起一定作用的糖分子。
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| 关 键 词： | 南方鲇(Silurus meridionalis) 凝集素 理化性质 |
| 文章编号： | 1000-6907-（2006）06-0029-04 |
| 收稿时间： | 2006-05-17 |
| 修稿时间： | 2006-05-17 |
Study on an Agglutinin Isolated from Serum of Silurus meridionalis |
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| ZHANG Li,YUAN Yuan,WANG Juan,GU Ji-rui,WU Jiang,XIE Xian-lian,XU Heng. Study on an Agglutinin Isolated from Serum of Silurus meridionalis[J]. Freshwater Fisheries, 2006, 36(6): 29-32 |
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| Authors: | ZHANG Li YUAN Yuan WANG Juan GU Ji-rui WU Jiang XIE Xian-lian XU Heng |
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| Abstract: | A natural agglutinin,which was isolated from serum of Silurus meridionalis,was found to have the ability of agglutinating different kinds of antigens. The agglutinin,of which the composition was glycoprotein, was stable when exposure to the heat for 1h and acid or alkaline for 24 h respectively,but it was sensitive to the treatment of β-mercaptoethanol. Its agglutinating activity was influenced by the temperature, reaction time and concentrating of ion of the buffer. The agglutinating activity could be inhibited by the splitting of gulactose and sucrose.The results demonstrated the galactose and sucrose played important roles in agglutinating reaction. |
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| Keywords: | Silurus meridionalis agglutinin physical and chemical characteristics |
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