| 农杆菌介导犁苞滨藜NHX基因转化苜蓿的影响因素 |
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| 引用本文： | 赵红娟,张博,李培英,陈爱萍. 农杆菌介导犁苞滨藜NHX基因转化苜蓿的影响因素[J]. 草地学报, 2007, 15(5): 418-422. DOI: 10.11733/j.issn.1007-0435.2007.05.004 |
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| 作者姓名： | 赵红娟 张博 李培英 陈爱萍 |
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| 作者单位： | 新疆农业大学草业工程学院, 新疆草地资源与生态自治区重点实验室, 新疆乌鲁木齐, 830052 |
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| 基金项目： | 新疆维吾尔自治区教育厅高校重点研究项目 |
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| 摘 要： | 以新牧1号杂花苜蓿(Medicago varia Martin.cv.Xinmu No.1)和新疆大叶苜蓿(Medicago sativa L.cv.Xinjiang Daye)无菌苗叶片和子叶为材料,通过根癌农杆菌EHA105介导转化犁苞滨藜NHX耐盐基因,提高植株耐盐性。本文对农杆菌转化条件进行研究,结果表明:以预培养3d的子叶为转化受体,菌液浓度OD600为0.4～0.5时,浸染10～15min,然后转入附加20～30mg/L乙酰丁香酮的共培养基,培养4d,可以获得较高的转化效率;对抗性芽的PCR检测初步证明,外源基因整合到苜蓿的基因组中。
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| 关 键 词： | 苜蓿 农杆菌 NHX基因 遗传转化 |
| 文章编号： | 1007-0435（2007）05-0418-05 |
| 收稿时间： | 2007-06-04 |
| 修稿时间： | 2007-07-03 |
Influencing Factors on Alfalfa Transformation with Atriplex dimorphostegia NHX Gene Mediated by Agrobacterium tumefaciens |
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| ZHAO Hong-juan,ZHANG Bo,LI Pei-ying,CHEN Ai-ping. Influencing Factors on Alfalfa Transformation with Atriplex dimorphostegia NHX Gene Mediated by Agrobacterium tumefaciens[J]. Acta Agrestia Sinica, 2007, 15(5): 418-422. DOI: 10.11733/j.issn.1007-0435.2007.05.004 |
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| Authors: | ZHAO Hong-juan ZHANG Bo LI Pei-ying CHEN Ai-ping |
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| Affiliation: | Xinjiang Key Laboratory of Grassland Resources and Ecology, College of Grassland Science, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China |
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| Abstract: | The leaves and cotyledons of Medicago varia Martin cv. Xinmu No.1 and Medicago sativa L. cv. Xinjiang Daye were used as materials and influencing factors on the salt-tolerant gene (NHX) transformation of Atriplex dimorphostegia Kar. et. Kir. by Agrobacterium tumefaciens EHA105 were studied. The results showed that the optimum transformation condition for cotyledon precultured 3 days and bacterial concentration of OD600 0.4~0.5 was immersing for 10~15 minutes, then laying cotyledons in co-culture medium with addition of 20~30 mg/L acetosyringone for 4 days. The PCR analysis showed that the NHX gene had been integrated into the genome of alfalfa. |
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| Keywords: | Alfalfa Agrobacterium tumefaciens NHX gene Genetic transformation |
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