不同采集时间及温度处理对花绒寄甲卵孵化率的影响

为了明确不同采集时间及温度处理对花绒寄甲卵孵化率的影响，采集时间设间隔1d、3d、5d，在8℃保存前后进行或不进行15℃缓冲，设4种温度处理类型共12种处理组合，对花绒寄甲卵的孵化率进行研究。结果显示：采集时间为间隔5d的孵化率或显著高于1d、3d，或与1d、3d无显著性差异。花绒寄甲卵8℃保存前后进行或不进行15℃缓冲，对其孵化率的影响无显著性差异，采集时间为间隔1d、3d或5d均是如此。综合本研究结果，考虑花绒寄甲卵卡实际生产过程中的简便性和可操作性，可考虑每隔5d收集卵一次，平均孵化率最高可达97．77％，校正孵化率为82．73％，8℃保存前后是否需要进行15℃缓冲处理，可视具体试验需求而定。

Effects of Different Collecting Times and Different Temperature Treatments on Egg Hatchabilities of Dastarcus helophoroides

In order to clarify the effects of different collecting times and different temperature treatments on egg hatehabilities of Dastarcus helophoroides （Coleoptera ： Bothrideridae）, collecting times of the eggs were set with 1-day, 3-day and 5-day intervals respectively, with or without 15℃ buffering treatments before and after the preservation of the eggs at 8℃. 4 types of temperature treatment were set with 12 treatment combinations to study their hatchabilities. The results showed that： For the 4 types of temperature treatment, the hatehability of the eggs with 5-day-interval collecting time was significantly higher than that of the eggs with 1-day and 3-day-interval collecting times, or no significant differences. There were no significant differences between hatehabilities of the eggs with or without 15 ℃ buffering treatments before and after their prservation at 8℃, including collecting times with 1-day, 3-day and 5-day intervals. From the results of the study and for the convenience and operability in real production process of D. helophoroides egg cards, the eggs could be collected with 5-day intervals, whose average hatchability could reach 97.77% at the most, and the corrected hatchability could be 82.73%. Specific test requirements may or may not require the 15℃ buffering treatments before and after the preservation of the eggs at 8℃.