Comparison of porcine reproductive and respiratory syndrome virus growth in media supplemented with fetal bovine serum or a serum replacement. |
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| Authors: | S K Schommer S L Carpenter P S Paul |
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| Affiliation: | Department of Veterinary Microbiology and Preventive Medicine, Veterinary Medical Research Institute, College of Veterinary Medicine, Iowa State University, Ames 50011, USA. |
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| Abstract: | Commercially available serum replacements are often used in cell culture as a cheaper and less variable substitute for fetal bovine serum (FBS). The growth of porcine reproductive and respiratory syndrome virus (PRRSV) isolates in CRL11171 cells maintained in a medium supplemented with FBS was compared with virus propagation in the same cell line maintained in the same medium with a serum replacement. The PRRSV replicated significantly better when the cell culture medium was supplemented with FBS. The results of this study have implications for the use of serum replacement-supplemented medium for PRRSV diagnosis by virus isolation. |
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