马实验性软组织伤冷冻效应观察

选择营养中等的成龄健康蒙古马10匹,分为两组,均实验性地造成体表软组织伤(长12cm,深3cm切口),分别立即及24h后进行冷冻。对冷冻诱起马体表局部软组织坏死及其修复特点,以及冷冻性免疫作用进行了系统观察。结果:①体表软组织冷冻性坏死的性质,最初属凝固性坏死,以后呈干性或湿性坏疽变化;②在冷冻性损伤修复过程中,损伤深部健康组织首先形成肉芽组织分界面,成为防止感染的屏障和修复损伤的肉芽组织基础;在坏死组织脱落后,伤面pH为7.0～7.7,有利于肉芽再生和防止化脓性感染;修复过程明显见有组织坏死及肉穿组织生成,属第二期愈合,但愈合后不留功能障碍;③冷冻性坏死能诱起T淋巴细胞显著增多(冷冻后第三周t=2.8068,P<0.05,第四周t=6.4869, P<0.01),可作为冷冻性免疫力增强的指标之一。

OBSERVATIONS OF THE EFFECTS OF CRYOCHEM ON THE EXPERIMENTAL WOUND OF SOFT TISSUE IN HORSE

Ten Mongolian horses in moderate nutrient condition wereused for systematically studying the effects of cryochem on the wound ofsurface soft tissues. The results were as follows: (1) The frozen necrosis ofsoft tissues was coagulative in nuture at first, and then became dry or moistgangrens; (2) During the repair of frozen wound, a demarcation ofgranulation was formed primarily, which served as a barrier againstinfection and as base of granulation for repairing. When necvrosis sloughed,the granulation has a pH range of 7.0--7.7. This pH is of advantage togranulation and disadvantage to pyogenic infection. The wound was healedby second intention for it had marked necrosis and granulation, but withoutfunctional hindrance; (3)Frozen necrosis could elevate T lymphocyte in blood(after 3 weeks, t = 2.8068, P<0.05; after 4 weeks, t = 6. 4869, P<0.01)which may be used as an indication of increased immunity.