Effect of pooling udder skin wipes on the detection of influenza A virus in preweaning pigs |
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Authors: | Anne C. de Lara Jorge Garrido-Mantilla Gustavo Lopez-Moreno My Yang David E.S.N. Barcellos Montserrat Torremorell |
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Affiliation: | Universidade Federal do Rio Grande do Sul, Faculdade de Veterinária, Porto Alegre, RS, Brazil;Pipestone Veterinary Services, Pipestone, MN, USA;Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, USA |
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Abstract: | Influenza A virus (IAV) active surveillance in pigs prior to weaning is commonly conducted by collecting individual samples, mostly nasal swabs. Recently, the use of udder skin wipes collected from lactating sows was identified as an effective sampling method to indicate IAV status of suckling piglets prior to weaning. However, there is limited information on the effect of pooling multiple udder wipes on the ability to detect IAV. We evaluated the effect of pooling 3, 5, or 10 udder wipes on the sensitivity of detecting IAV and compared the results with testing the wipes individually. The likelihood of detecting positive udder wipes decreased with pooling when the initial positive cycle threshold value was ≥31.5; pooling of up to 3 samples could be performed without affecting sensitivity significantly. Our results support pooling of udder skin wipes to conduct surveillance of IAV in pigs prior to weaning. |
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Keywords: | influenza A virus pigs pooling surveillance udder wipes |
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