银线草HPLC指纹图谱研究

目的]建立银线草HPLC指纹图谱研究方法,为其质量控制提供依据。方法]采用高效液相色谱法,色谱柱为Phenomenex C18(250.0 mm ×4.6 mm,5.0μm)；流动相甲醇-0.1%甲酸线性梯度洗脱,流速为0.8 mL/min；检测波长323 nm；柱温25℃。结果]建立了银线草HPLC指纹图谱共有模式,并标定12个共有峰,13批样品指纹图谱相似度在0.714~0.957。结论]不同产地的银线草化学成分存在一定差异,所建立的指纹图谱方法简单、准确可靠、重复性好,为银线草质量标准的制定提供科学依据。

HPLC Fingerprint of Chloranthus japonicus Sieb.

Objective] To establish a method of HPLC fingerprint analysis on Chloranthus japonicus Sieb. , and to provide references for the quality control. Method] High-performance liquid chromatography was adopted. The chromatographic column was Phenomenex C18 (250. 0 mm × 4. 6 mm, 5. 0 μm);mobile phase was methanol-0. 1% formic acid with linear gradient elution; flow rate was 0. 8 mL/min;detection wavelength was 323 nm and the column temperature was maintained at 25℃. Result] HPLC fingerprint of C. japonicus was estab-lished. Twelve main marker peaks were selected. Fingerprint similarities of 13 sample batches were distributed between 0. 714 and 0. 957. Conclusion] There are certain differences in the chemical components of C. japonicus in different production areas. The established finger-print method is simple, accurate, reliable and repeatable, which provides scientific references for the quality standard establishment of C. ja-ponicus.