响应面法优化费菜SOD提取条件的研究

目的]利用响应面法优化费菜SOD的提取条件。方法]首先进行单因素优化试验,单因素分别为时间、p H、液料比。经过单因素优化试验,得出最优时间、最优p H、最优料液比。通过Design expert8.0.5软件设计出17种试验方案。通过试验,得出每种方案的蛋白得率,利用Design expert8.0.5软件进行了方差分析,各因素之间的交互作用分析,并得出了相应的数学模型。结果]单因素试验中,最优时间为1 h,最优p H为7.8、最优料液比为1∶15 g/m L。各因素交互作用分析表明,提取费菜SOD的最佳方案为提取时间0.98 h、p H7.79、料液比1∶13.71 g/m L。利用邻苯三酚自氧法测得提取的SOD酶活为12 U/g。结论]该研究结果可为费菜资源的进一步利用与研究提供参考。

Study on Optimizing Extracting Conditions of SOD in Sedum aizoon by the Response Surface Method

Objective] The aim was to optimize extracting conditions of SOD in Sedum aizoon by response surface method.Method] First, the single factor optimization tests were carried out.Three single factors are time, pH and solid-to-liquid ratio respectively.By Design expert 8.0.5 software, 17 kinds of test scheme were designed.The protein yield of each scheme was obtained, Design expert 8.0.5 software was used to con-duct variance analysis, interactive analysis among factors, corresponding mathematical model was obtained.Result] The results showed that in single factor experiments, the optimal time is 1 hour, pH is 7.8, and solid-to-liquid ratio is 1∶15 g/mL.Factors interactive analysis indicated that the optimal scheme for extracting SOD in Sedum aizoon is time 0.98 h, pH 7.79, solid-to-liquid ratio 1∶13.71 g/mL.By the method of py-rogallol autoxidation, the extracted SOD enzyme activity was measured, that is 12 U/g.Conclusion] The results can provide reference for fur-ther utilization and research of Sedum aizoon resources.