生石花种子萌发及幼苗生长最优条件的筛选

目的]优化生石花种子萌及幼苗生长的条件,以提高其种子萌发率。方法]采用MS、1/2MS、3/4MS等培养基为生石花生长基质,比较不同培养基中种子萌发率和幼苗生长情况,在此基础上进行光照时间、激素配比和浸泡时间的筛选。结果]1/2MS培养基最适合生石花种子萌发及幼苗生长。16 h为最优光照时间;不同因素影响由大到小依次为浸泡时间、GA浓度、IAA浓度、NAA浓度及6-BA浓度。其中,GA浓度与浸泡时间对种子萌发有显著影响,且0.1 mg/m L的GA浓度以及4 h的浸泡时间为最优组合。结论]该研究为生石花组织培养快繁技术研究奠定基础。

Screening of Different Mediums and Hormone Concentrations for Seed Germination and Growth Conditions of Lithops

Objective] To optimize the seed germination condition of Lithops, and to enhance the germination rate of seeds using MS culture medium to replace soil as growth medium of Lithops.Method] Compared with the seed germination rate, root growth and seedling growth in different culture mediums, we screened the illumination time, hormone proportion and soaking time.Result] 1/2 MS culture medium is the most suitable culture medium for the germination and growth of Lithops seeds.Illumination for 16 h was the optimal time.The factors influen-cing degree from big to small was soaking time, GA concentration, IAA concentration and 6-BA concentration.Among them, GA concentra-tion and soaking time had significant effects on seed germination.And 0.1 mg/mL GA and 4 h soaking time were the optimal combination. Conclusion] This research provides basis for the rapid propagation technique of Lithops tissue culture.