Antifungal activity of thiophenes from Echinops ritro |
| |
Authors: | Fokialakis Nikolas Cantrell Charles L Duke Stephen O Skaltsounis Alexios L Wedge David E |
| |
Affiliation: | Natural Products Utilization Research Unit, Agricultural Research Service, U.S. Department of Agriculture, P.O.Box 8048, University of Mississippi, University, Mississippi 38677, USA. |
| |
Abstract: | Extracts from 30 plants of the Greek flora were evaluated for their antifungal activity using direct bioautography assays with three Colletotrichum species. Among the bioactive extracts, the dichloromethane extract of the radix of Echinops ritro (Asteraceae) was the most potent. Bioassay-guided fractionation of this extract led to the isolation of eight thiophenes. Antifungal activities of isolated compounds together with a previously isolated thiophene from Echinops transiliensis were first evaluated by bioautography and subsequently evaluated in greater detail using a broth microdilution assay against plant pathogens Colletotrichum acutatum, Colletotrichum fragariae, Colletotrichum gloeosporioides, Botrytis cinerea, Fusarium oxysporum, Phomopsis viticola, and Phomopsis obscurans. 5'-(3-Buten-1-ynyl)-2,2'-bithiophen (1), alpha-terthienyl (2), and 2-[pent-1,3-diynyl]-5-[4-hydroxybut-1-ynyl]thiophene (5) at 3 and 30 microM were active against all three Colletotrichum species, F. oxysporum, P. viticola, and P. obscurans. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|