| 结构域Tt APuX21基因的分离与克隆研究 |
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| 引用本文： | 谢晚彬.结构域Tt APuX21基因的分离与克隆研究[J].湖北农业科学,2008,47(5):493-495. |
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| 作者姓名： | 谢晚彬 |
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| 作者单位： | 宜春学院化学与生物工程学院,江西,宜春,336000 |
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| 基金项目： | 江西省宜春市科技计划
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宜春学院校级科研项目 |
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| 摘 要： | 以热产硫化氢高温厌氧杆茵茵液为模板,根据Gene Bank中登记的编码结构域Tt APuX21基因序列设计1对特异引物,利用多聚酶链反应技术,扩增出目的基因Tt apux21,并克隆到表达栽体pET21a( )中.经茵液PCR筛选和DNA测序鉴定.表达栽体pET21a( )中插入有序列正确的Tt apux21基因,重组质粒命名为pEX21.
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| 关 键 词： | 结构域 PCR扩增 克隆 |
Study on Isolation and Cloning of the Gene Encoding Module Tt APuX21 |
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| XIE Wan-bin.Study on Isolation and Cloning of the Gene Encoding Module Tt APuX21[J].Hubei Agricultural Sciences,2008,47(5):493-495. |
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| Authors: | XIE Wan-bin |
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| Institution: | XIE Wan-bin(College of Chemistry , Bioengineering,Yichun University,Yichun 336000,Jiangxi,China) |
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| Abstract: | A pair of specific primers were designed according to the gene sequence encoding module Tt APuX21 adopted in Gene Bank.By the improved PCR amplification technology,interest gene Tt apux21 was obtained from Thermoanaerobacter thermohydrosulfuricus,and then cloned into expression vector pET21a(+).Bacterial culture PCR showed that interest gene Tt apux21 had been inserted into pET21a(+),and DNA sequencing revealed that the sequence of the inserted fragment was the same as that of Tt apux21 adopted in Gene Bank... |
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| Keywords: | module PCR amplification cloning |
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