纳米碳增效剂对柑橘产量及土壤细菌种群结构的影响

为揭示新型纳米碳增效剂对柑橘产量的影响及其机理和研究对土壤微生物种群结构影响,本研究以三峡库区万州柑橘园为对象,以常规施肥为对照(ZJ1),采用正交优化实验确定柑橘产量的最佳施用量,同时基于Hiseq 2500高通量测序方法,分析不同施肥处理条件下的土壤细菌种群多样性。研究结果发现,柑橘产量的最佳施肥组合为ZJ5(A_2B_1C_2);微生物多样性受复合肥用量影响最大,纳米碳次之,腐植酸保水剂影响最小。最佳施肥组合(ZJ5)放线菌门(Actinbacteria)丰度最高,疣微菌门(Verrucomicrobia)丰度最低;通过对环境因子的分析发现,影响微生物种群多样性变化的环境因素顺序为p H有机质水解性氮。

Effects of super absorbent polymer of nano carbon on citrus yield and soil bacterial communities structure

In order to reveal the effect of super absorbent polymer of nano carbon and soil microbial community structure on citrus yield,field experiment was conducted in the citrus orchard in Wanzhou,the Three Gorges Reservoir Area.Conventional fertilization(ZJ1)was used as the control.The optimum application rate of Nano carbon(A),Aquasorb fertilizer(B)and compound fertilizer(C)on citrus yield was determined by orthogonal experiment.Simultaneously,soil bacterial community structure under different fertilization treatments were analyzed based on Hiseq 2500 High throughput sequencing method.The best fertilization combination for citrus yield was(ZJ5)A2B1C2;the microbial diversity was most influenced by the compound fertilizer,followed by Nano carbon,and the effect of Aquasorb fertilizer was the minimum.It was found that the relative abundance of Actinbacteria was the highest in the optimum fertilization combination(ZJ5),and Verrucomicrobia was the lowest.It was found that the order of environmental factors which affecting the microbial community change were pH> organic> hydrolyzable nitrogen.