| 响应面法优化钝裂银莲花花色素提取工艺 |
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| 引用本文： | 蓝雯琳,刘左军,刘志鹏. 响应面法优化钝裂银莲花花色素提取工艺[J]. 草业科学, 2017, 34(1). DOI: 10.11829/j.issn.1001-0629.2016-0062 |
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| 作者姓名： | 蓝雯琳 刘左军 刘志鹏 |
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| 作者单位： | 兰州理工大学生命科学与工程学院,甘肃 兰州,730000 |
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| 摘 要： | 以钝裂银莲花(Anemone obtusiloba)的萼片为提取材料,选取料液质量浓度、pH、提取时间、提取温度4个因素,以钝裂银莲花花色素提取液的吸光值为响应面,对钝裂银莲花花色素的提取条件进行优化,用四舍五入法确定了钝裂银莲花花色素提取的最佳工艺为:料液质量浓度70g·mL~(-1),pH 3.8,提取温度61℃,提取时间87min,在此条件下,钝裂银莲花花色素提取液的吸光值可达最佳,为0.154 5。
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| 关 键 词： | 钝裂银莲花 花色素 优化 响应面法 |
Optimization of anthocyanidin extraction from Anemone obtusiloba using response surface methodology |
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| Lan Wen-lin,Liu Zuo-jun,Liu Zhi-peng. Optimization of anthocyanidin extraction from Anemone obtusiloba using response surface methodology[J]. Pratacultural Science, 2017, 34(1). DOI: 10.11829/j.issn.1001-0629.2016-0062 |
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| Authors: | Lan Wen-lin Liu Zuo-jun Liu Zhi-peng |
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| Abstract: | In this study,we used the sepals of Anemone obtusiloba as experimental material for extraction of anthocyanidin using response surface methodology.Four factors were selected in this experiment: the material liquid mass concentration,pH,extraction time,and extraction temperature.We selected the light absorption value of the anthocyanidin of A.obtusiloba as a response surface to optimize the extraction conditions of anthocyanidin.Our results confirmed that the most efficient extraction of anthocyanidin was achieved under the following conditions: a material liquid mass concentration of 70 g·mL-1,an extraction temperature of 61 ℃,an extraction time of 87 min,and pH 3.8.Under these conditions,we conclude that the best light absorption value of A.obtusiloba is 0.154 5. |
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| Keywords: | Anemone obtusiloba anthocyanin optimization response surface method |
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