沧州盐碱地紫花苜蓿根际促生细菌的筛选

本研究以河北省沧州黄骅市紫花苜蓿(Medicago sativa)为研究对象,对采自黄骅地区常郭乡赵子札村、羊二庄镇张八寨村及旧城镇云庄村等10个地点的紫花苜蓿根际土壤进行细菌分离纯化和培养,获得134株根际细菌。进而将其接种到紫花苜蓿植物种苗,培养60d后,比较分析了接种不同根际促生菌对植物生长的促生影响,选择了30株促生细菌优良菌株,并且对30株细菌菌株进行了溶磷能力和分泌生长素能力的测定。结果表明,菌株EF2对植物干重增重影响最为显著(P0.05),其溶解有机磷和无机磷能力也最强;菌株LF13分泌生长素的能力最强,其对株高的影响较为明显。研究最终初步筛选出9株溶磷能力和分泌生长素能力较强并能促进紫花苜蓿显著生长的根际细菌。

Screening of plant growth-promoting bacteria in alfalfa grownin saline in Cangzhou,Hebei Province

This study focused on Medicago sativa growing in Huanghua City,Cangzhou,Hebei Province.Soils adhered to M.sativa roots were sampled from10 different locations in Huanghua City,including Zhaozizha Vil-lage in Changguo,Zhangbazhai Village in Yangerzhuang,and Yunzhuang Village in Jiucheng Town.In total, 134 strains were obtained by isolation and cultivation from soil samples.Of these strains,30 were chosen based on the growth promotion of M.sativa seedlings after 60 days of incubation.We also compared the performance of the strains based on their phosphate solubilization and auxin secretion abilities.The result showed that the strain EF2 had a significant positive influence on plant dry weight,and it also had the strongest phosphate solu-bilization ability,whereasthe strain LF13 hadthe strongestinfluenceon plant height,and also had the strongest auxin secretion ability.In conclusion,we obtained nine highly effective strains by investigating the ability of phosphate solubilization and auxin secretion,as well as growth promotion.