不同pH下接种AM真菌和根瘤菌对紫花苜蓿生长的影响

本研究设定4个pH梯度(4.43,5.06,6.43和7.16),设置了接种AMF根内球囊霉(Glomus intraradices)、苜蓿中华根瘤菌(Sinorhizobium medicae)、同时接种AMF和根瘤菌以及不接菌处理分析温室不同pH处理下接种丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)和根瘤菌(rhizobia)对紫花苜蓿(Medicago sativa)生长的影响。结果表明,酸胁迫抑制了紫花苜蓿的生长,使其株高降低了1.27%~10.13%,地上生物量降低了33.33%~55.56%;AM真菌可以缓解酸胁迫,促进紫花苜蓿的生长,与不接种AM真菌相比,株高提高了244.26%~294.17%,地上生物量提高了727.78%~1 737.50%,地下生物量提高了1 120%~2 220%,根长提高了39.85%~102.63%,根冠比提高了5.71%~90.32%;根瘤菌单独接种未显著影响紫花苜蓿生长(P0.05),但与AM真菌混合接种后,可显著促进紫花苜蓿生长(P0.05)。

Effects of inoculation with arbuscular mycorrhizal fungi and rhizobia on growth of Medicago sativa in acidic soil

Experiments were performed to test the effects of arbuscular mycorrhizal fungi (AMF) and rhizobia on the growth of Medicago sativa (alfalfa) with a variety of soil pH in a greenhouse.Plants were inoculated with Glomus intraradices (AMF), Sinorhizobium medicae (rhizobium), both, or neither, under four different soil pH treatments (4.43, 5.06, 6.43, and 7.16).Acid stress inhibited alfalfa growth, such that plant height was decreased 1.27%~10.13% and biomass decreased 33.33%~55.56%.AMF alleviated acid stress and promoted alfalfa plant height by 244.26%~294.17%, shoot biomass by 727.78%~1 737.50%, root biomass by 1 120%~2 220%, root length by 39.85%~102.63%, root: shoot ratio by 5.71%~90.32%.Inoculation with rhizobia alone did not significantly affect alfalfa growth(P>0.05), but the co-inoculation of AMF and rhizobia significantly increased alfalfa growth(P<0.05).