黄花草木樨水浸提液对苏丹草和黑麦草的化感作用

黄花草木樨水浸提液具有化感除草作用,为了进一步开展黄花草木樨化感抑草的研究,阐明黄花草木樨中所含化感物质的化感抑制机理,本实验采用生物检测法以黄花草木樨水浸提液处理多花黑麦草和苏丹草种子,并对其萌发和幼苗生长过程中的重要生理生化指标进行分析测定。结果表明,与对照相比,黄花草木樨水浸提液能显著降低多花黑麦草和苏丹草种子的发芽率、根长、根干重、茎长和茎叶干重等生长指标(P<0.05);能显著降低多花黑麦草和苏丹草的叶绿素含量(P<0.05),显著提高多花黑麦草和苏丹草的淀粉和可溶性蛋白含量(P<0.05);显著提高苏丹草可溶性糖含量,显著降低黑麦草可溶性糖含量(P<0.05);苏丹草和黑麦草在4和6 d处理组的CAT活性与对照差异显著(P<0.05),8 d时恢复至对照水平;苏丹草处理组和黑麦草处理组的POD活性均先降低后升高。

Allelopathic effects of an aqueous extract of Melilotus officinalis on Lolium multiflorum and Sorghum sudanense

The aqueous extract of Melilotus officinalis is known to inhibit weed growth.To study in detail the allelopathic inhibition of grass growth and elucidate the allelopathic mechanism,an aqueous extract of M.officinalis was applied to seeds and seedlings of Lolium multiflorum and Sorghum sudanense and its effects on their growth were evaluated by measuring several important physiological and biochemical indexes.The aqueous extract of M.officinalis significantly (P＜0.05) reduced the germination rate,root length,root dry weight,stem length,stem and leaf dry weight of both test species,compared with their respective controls.The aqueous extract of M.officinalis significantly reduced the chlorophyll content and increased the starch and soluble protein contents (P＜0.05) of both test species.Treatment with the aqueous extract of M.officinalis significantly (P＜0.05) increased the soluble sugars content in S.sudanense and significantly (P＜0.05) reduced the soluble sugars content in L.multiflorum.The catalase activity in S.sudanense treated with the M.officinalis extract differed significantly (P＜0.05) from that of its control,but returned to control levels after 8 days.The peroxidase activity in S.sudanense treated with the M.officinalis aqueous extract first decreased and then increased.