免疫荧光层析快速检测河豚毒素的研究

本研究利用自制的河豚毒素荧光免疫层析检测卡对河豚毒素进行快速定量检测,并分析其稳定性、灵敏度、趋势线拟合程度及检测范围。研究结果表明,自制的河豚毒素荧光免疫层析检测卡变异系数为0.124,拟合方程相关系数r~2值为0.995 5,检测卡的检测范围为2~600 ng/m L,可以满足对河豚毒素快速定量检测的要求。

The study of immunofluorescence chromatography detection in tetrodotoxin

In the southeast coastal area of China, tetrodotoxin poisoning often occurred for eating puffer fish. Currently main detection methods of tetrodotoxin are mouse assay and enzyme linked immunosorbent assay ( ELISA) etc. The tetrodotoxin detection methods presently need much more effort in time and cost, and can not meet the market requirements nowadays. We developed a tetrodotoxin colloidal gold immunochromato-graphic test kit, which the lowest detection limit was 100 ng/mL. The logic four parameter curve fitting, var-iation coefficient analysis, and standard test methods were used to test the stability, sensitivity, the fitting degree of the trend line, and detection range of homemade detection card in tetrodotoxin quantitative detec-ting. The results showed that the variation coefficient of homemade tetrodotoxin fluorescence immuno-chrom-atographic testing card was 0. 124; correlation coefficient r2 value was 0. 995 5; the detection range was 2~600 ng/mL. The testing card can meet the need of tetrodotoxin detection.