| 猪流行性腹泻病毒疫苗株与野毒株RT-PCR检测方法的建立与应用 |
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| 引用本文： | 朱海侠,曾亮明,傅星源,张蕴冰,陈雷,张渊魁. 猪流行性腹泻病毒疫苗株与野毒株RT-PCR检测方法的建立与应用[J]. 福建畜牧兽医, 2016, 0(3): 14-16. DOI: 10.3969/j.issn.1003-4331.2016.03.007 |
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| 作者姓名： | 朱海侠 曾亮明 傅星源 张蕴冰 陈雷 张渊魁 |
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| 作者单位： | 福州大北农生物技术有限公司 福州 350014 |
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| 基金项目： | 福州大北农生物技术有限公司畜禽常见病免疫防控技术研究专项基金资助。 |
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| 摘 要： | 根据GenBank中猪流行性腹泻(PED)疫苗株与野毒株ORF3的特点设计特异性引物,建立能够快速区分PEDV疫苗株与野毒株的RT-PCR检测方法。建立的RT-PCR检测方法能够对PEDV疫苗株与野毒株扩增出特异性片段,其大小分别为278 bp和327 bp。该方法具有快速、特异、通用等特点,可用于实验室快速诊断、野毒株的区分,为该病的诊断及免疫防控提供参考依据。
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| 关 键 词： | 猪流行性腹泻 疫苗株 野毒株 |
Establishment and application of RT-PCR assay for detection of PEDV vaccine and wild strains |
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| Abstract: | Based on the characteristics of porcine epidemic diarrhea (PED) gene in ORF3 between vaccine and wild strains, one pair of primers was designed and a rapid diagnostic method of RT-PCR was established. The established method had a rapid and general features and it only amplified target band from PEDV. A total of 278 bp bands were amplified from vaccine strains and 327 bp bands were amplified from wild strains. A highly special RT-PCR method was established and it can directly differentiate PEDV vaccine strains and wild strains, which can be provide reference for diagnosis and the immune prevention. |
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| Keywords: | Porcine epidemic diarrhea(PED) Vaccine strain Wild strain |
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